<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Christia vespertilionis (butterfly wing plant) is an ornamental plant originated from South East Asia with reported usage in traditional medicine practice and potential as an anticancer and antitumor. This research aims to estimate the genome size of C. vespertilionis via flow cytometry (FCM) method. The research was conducted with the optimisation of nuclear suspension preparation followed by the genome size estimation. Two chopping techniques [manual chopping (MC) and BDTM Medimachine (MM)] and two lysis buffers (Otto and LBO1) were tested. Otto buffer with manual chopping was found to be the most suitable method, generated fine DNA peak with minimum debris background, and coefficient of variation (CV) value less than 3%. Five replicates of the FCM analysis were made for the genome size determination. The estimated genome size of C. vespertilionis was found to be 3.22 pg by using Glycine max cv. Polanka (2C=2.5pg) as an external reference standard. Further comparison with other Christia species was not possible due to the lack of data on genome size. The genome size data of C. vespertilionis can be useful for future morphology and genetics studies of Christia species.</p></abstract>

Christia vespertilionis (butterfly wing plant) is an ornamental plant originated from South East Asia with reported usage in traditional medicine practice and potential as an anticancer and antitumor. This research aims to estimate the genome size of C. vespertilionis via flow cytometry (FCM) method. The research was conducted with the optimisation of nuclear suspension preparation followed by the genome size estimation. Two chopping techniques [manual chopping (MC) and BDTM Medimachine (MM)] and two lysis buffers (Otto and LBO1) were tested. Otto buffer with manual chopping was found to be the most suitable method, generated fine DNA peak with minimum debris background, and coefficient of variation (CV) value less than 3%. Five replicates of the FCM analysis were made for the genome size determination. The estimated genome size of C. vespertilionis was found to be 3.22 pg by using Glycine max cv. Polanka (2C=2.5pg) as an external reference standard. Further comparison with other Christia species was not possible due to the lack of data on genome size. The genome size data of C. vespertilionis can be useful for future morphology and genetics studies of Christia species.

Nuclear Genome Size Determination Of Christia Vespertilionis Via Flow Cytometry

Department of Plant Science, Kulliyyah of Science

International Islamic University Malaysia

Acta Chemica Malaysia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Nuclear Genome Size Determination Of Christia Vespertilionis Via Flow Cytometry"}

Christia vespertilionis (butterfly wing plant) is an ornamental plant originated from South East Asia with reported usage in traditional medicine practice and...

Nuclear Genome Size Determination Of Christia Vespertilionis Via Flow Cytometry

Data publikacji: 03 sie 2020

Zakres stron: 72 - 75

DOI: https://doi.org/10.2478/acmy-2020-0012

Słowa kluczowe
Christia vespertilionis, pokok rerama, flow cytometry, genome size

© 2020 Mohd Razik Midin et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Nuclear Genome Size Determination Of Christia Vespertilionis Via Flow Cytometry

Data publikacji: 03 sie 2020

Zakres stron: 72 - 75

DOI: https://doi.org/10.2478/acmy-2020-0012

Słowa kluczoweChristia vespertilionis, pokok rerama, flow cytometry, genome size

© 2020 Mohd Razik Midin et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
Christia vespertilionis, pokok rerama, flow cytometry, genome size