Using in vitro methods to estimate metabolizable energy content of five forage legumes harvested under different defoliation systems / Einsatz von in vitro Methoden zur Schätzung der umsetzbaren Energie in fünf Futterleguminosen aus unterschiedlichen Nutzungssystemen
, , , , oraz | 02 gru 2016
O artykule
Article Category: Research Article
Data publikacji: 02 gru 2016
Zakres stron: 121 - 131
Otrzymano: 22 kwi 2016
Przyjęty: 20 lip 2016
DOI: https://doi.org/10.1515/boku-2016-0011
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© 2016 De Gruyter, www.degruyter.com/view/j/boku
This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Differences between the in vitro analyzed OMD (CM-OMD: pepsin-cellulase method; TT-OMD: Tilley and Terry method) of four standard samples and their in vivo digestibility; TT-OMD data prior to routine correction with in vivo OMDTabelle 2. Differenzen der in vitro untersuchten OMD (CM-OMD: Pepsin-Cellulase-Methode; TT-OMD: Tilley und Terry-Methode) für vier Standardproben zu in vivo Verdaulichkeit; TT-OMD Daten vor der Routinekorrektur durch die in vivo OMD
| Standard samples | CM-OMD (%) | TT-OMD (%) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Range Minimum and maximum differences to the | Range Minimum and maximum differences to the | ||||||||||||
| No. | Cuts/year | Cut | (%) | Number of runs in which standard samples was included | Mean Mean of the differences to the | SD Standard deviation of the differences of the laboratory-determined values to the | Mean Mean of the differences to the | SD Standard deviation of the differences of the laboratory-determined values to the | |||||
| Min | Max | Min | Max | ||||||||||
| 198 | 1 | 1 | 41.9±3.92 | 5 | 0.72 | 3.30 | 2.06 | 1.13 | 24 | −15.44 | 1.89 | −2.44 | 3.99 |
| 232 | 3 | 1 | 68.4±2.13 | 5 | −3.91 | −2.88 | −3.63 | 0.43 | 24 | −10.82 | 1.09 | −3.45 | 3.16 |
| 246 | 4 | 2 | 75.2±2.26 | 4 | 2.92 | 4.34 | 3.78 | 0.63 | 24 | −7.74 | 0.93 | −3.50 | 3.07 |
| 298 | 1 | 1 | 46.7±4.15 | 5 | 2.65 | 3.65 | 2.98 | 0.40 | 23 | −15.56 | 1.22 | −3.20 | 4.68 |
ME contents of several forage legumes from the 3-cut system at three cutting dates over study sites and years, based on two different in vitro methods (n = 286), expressed as MJ ME/kg DMTabelle 3. ME Gehalt verschiedener Futterleguminosen (3-Schnittsystem) als Mittelwert über Standorte und Jahre, mittels zwei in vitro Methoden (n = 286), als MJ ME/kg TS
| Pepsin-cellulase method (MECM) | Tilley and Terry method (METT) | Means over methods | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cut 1 | Cut 2 | Cut 3 | Cut 1 | Cut 2 | Cut 3 | Cut 1 | Cut 2 | Cut 3 | |||
| White clover | 11.0 | 10.1 | 10.6 | 10.6 v,V | 10.1 | 8.8 | 9.4 | 9.4 w,V | 10.5 a,A | 9.4 a,C | 10.0 a,B |
| Red clover | 10.3 | 9.7 | 10.0 | 10.0 v,W | 9.2 | 8.2 | 8.8 | 8.7 w,W | 9.8 b,A | 8.9 b,C | 9.4 b,B |
| Lucerne | 9.9 | 9.5 | 9.5 | 9.6 v,X | 8.3 | 7.6 | 7.8 | 7.9 w,X | 9.1 c,A | 8.5 c,B | 8.6 c,B |
| Birdsfoot trefoil | 9.9 | 9.3 | 9.4 | 9.5 v,X | 8.5 | 7.6 | 7.5 | 7.9 w,X | 9.2 c,A | 8.5 c,B | 8.4 d,B |
| Mean | 10.3 g,G | 9.6 g,I | 9.9 g,H | 9.9 | 9.0 h,G | 8.1 h,I | 8.4 h,H | 8.5 | |||
ME contents of forage legumes from different defoliation systems (3-cut system, 3C; 5-cut system, 5C; rotational grazing, RG) from the site in Germany over years, estimated by NIRS based on two different in vitro methods, expressed as MJ ME/kg DMTabelle 4. ME Gehalte von Futterleguminosen verschiedener Nutzungssystems (3-Schnitt-, 3C; 5-Schnittsystem, 5C; Umtriebsweide, RG) aus dem Standort Deutschland als mittel über Jahre, geschätzt mittels NIRS aus zwei unterschiedlichen in vitro Methoden, als MJ ME/kg TS
| Pepsin-cellulase method (MECM) | Tilley and Terry method (METT) | Means over methods | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cut 1 | Cut 2 | Cut 3 | Cut 4 | Cut 5 | Cut 1 | Cut 2 | Cut 3 | Cut 4 | Cut 5 | Cut 1 | Cut 2 | Cut 3 | Cut 4 | Cut 5 | |||
| 3-cut system ( | |||||||||||||||||
| WC | 11.0 | 10.0 | 10.2 | 10.4 v,V | 10.6 | 8.8 | 9.3 | 9.6 w,V | 10.8 a,A | 9.4 a,C | 9.8 a,B | ||||||
| RC | 10.4 | 9.7 | 9.4 | 9.8 v,W | 9.6 | 8.3 | 8.6 | 8.8 w,X | 10.0 c,A | 9.0 b,B | 9.0 b,B | ||||||
| KC | 10.8 | 10.3 | 10.3 | 10.5 v,V | 9.9 | 9.0 | 8.9 | 9.2 w,W | 10.3 b,A | 9.6 a,B | 9.6 a,B | ||||||
| LC | 9.8 | 9.5 | 9.0 | 9.4 v,X | 8.4 | 7.7 | 7.4 | 7.8 w,Y | 9.1 e,A | 8.6 c,B | 8.2 c,C | ||||||
| BT | 10.0 | 9.7 | 8.9 | 9.6 v,X | 9.1 | 7.9 | 7.0 | 8.0 w,Y | 9.6 d,A | 8.8 bc,B | 8.0 c,C | ||||||
| 10.4 g,G | 9.8 g,H | 9.6 g,I | 9.9 | 9.5 h,G | 8.3 h,H | 8.2 h,H | 8.7 | ||||||||||
| 5-cut system ( | |||||||||||||||||
| WC | 11.8 | 11.1 | 9.8 | 11.1 | 11.2 | 11.0 v,V | 10.1 | 9.9 | 8.6 | 9.7 | 9.5 | 9.5 w,V | 10.9 a,A | 10.5 a,B | 9.2 b,C | 10.4 a,B | 10.4 a,B |
| RC | 11.2 | 10.8 | 9.9 | 10.4 | 10.7 | 10.6 v,X | 9.6 | 9.5 | 8.4 | 8.8 | 8.6 | 9.0 w,X | 10.4 b,A | 10.1 b,B | 9.1 b,D | 9.6 c,C | 9.6 c,C |
| KC | 11.4 | 10.9 | 10.5 | 10.9 | 10.8 | 10.9 v,W | 9.6 | 9.5 | 9.0 | 9.4 | 9.2 | 9.3 w,W | 10.5 b,A | 10.2 b,B | 9.7 a,C | 10.1 b,B | 10.0 b,B |
| LC | 10.8 | 10.4 | 10.0 | 10.1 | 10.4 | 10.4 v,Y | 8.8 | 8.6 | 8.0 | 8.2 | 8.2 | 8.4 w,Y | 9.8 c,A | 9.5 c,B | 9.0 b,D | 9.2 d,CD | 9.3 d,BC |
| BT | 10.9 | 9.9 | 9.8 | 10.1 | 10.4 | 10.2 v,Z | 8.5 | 7.9 | 7.6 | 7.9 | 8.0 | 8.0 w,Z | 9.7 c,A | 8.9 d,C | 8.7 c,D | 9.0 e,C | 9.2 d,B |
| 11.2 g,G | 10.6 g,HI | 10.0 g,J | 10.5 g,I | 10.7 g,H | 10.6 | 9.3 h,G | 9.1 h,H | 8.3 h,J | 8.8 h,I | 8.7 h,I | 8.8 | ||||||
| RG ( | |||||||||||||||||
| WC | 11.5 | 10.9 | 10.6 | 11.2 | 11.5 | 11.1 v,V | 10.5 | 9.7 | 9.5 | 9.7 | 9.4 | 9.8 w,V | 11.0 a,A | 10.3 a,B | 10.0 a,C | 10.5 a,B | 10.4 a,B |
| RC | 11.1 | 11.1 | 10.1 | 10.5 | 10.6 | 10.7 v,W | 9.6 | 9.6 | 8.8 | 8.7 | 8.7 | 9.1 w,W | 10.4 b,A | 10.4 a,A | 9.5 b,B | 9.6 b,B | 9.6 b,B |
| LC | 10.6 | 10.5 | 10.4 | 10.2 | 10.4 | 10.4 v,X | 8.7 | 8.8 | 8.7 | 8.2 | 8.1 | 8.5 w,X | 9.6 c,A | 9.7 b,A | 9.6 b,A | 9.2 c,B | 9.2 c,B |
| BT | 9.9 | 10.2 | 9.7 | 10.1 | 10.4 | 10.1 v,Y | 8.2 | 8.3 | 7.8 | 7.7 | 8.1 | 8.0 w,Y | 9.1 d,AB | 9.3 c,A | 8.7 c,C | 8.9 d,BC | 9.3 c,A |
NIRS calibration statistics of the determined parameters IOM (g/kg DM), MECM (MJ/kg DM), TT-OMD (%), and METT (MJ/kg DM) of the legume samples (n = 431)Tabelle 1. NIRS Kalibrationsstatistik für folgende Parameter: IOM (g/kg TM), MECM (MJ/kg TM), TT-DOM (%) und METT (MJ/kg TM)
| Parameter | Number of samples included in the calibration | Range Minimum and maximum of the parameter values | Mean Mean of the parameter values | SD Standard deviation of the laboratory-determined values | SEC Standard error of calibration | R2 Coefficient of determination; relationship between NIRS- and laboratory-determined values | CVSD Variation coefficient referred to the SD of the reference method ( | |
|---|---|---|---|---|---|---|---|---|
| IOM IOM, enzymatically insoluble organic matter; MECM, ME content estimated with pepsin-cellulase method; TT-OMD, Tilley and Terry method estimated organic matter digestibility; METT, ME content estimated using Tilley and Terry method data | 77 | 90.60 | 353.73 | 204.42 | 72.18 | 11.52 | 0.98 | 15.96 |
| MECM | 76 | 8.18 | 11.90 | 10.11 | 0.93 | 0.15 | 0.97 | 16.27 |
| TT-OMD | 77 | 42.42 | 79.83 | 63.51 | 7.82 | 3.24 | 0.83 | 41.43 |
| METT | 73 | 6.20 | 11.03 | 8.61 | 1.05 | 0.59 | 0.69 | 55.86 |