<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Mechanotransduction is of fundamental importance in cell physiology, facilitating sensing in touch and hearing as well as tissue development and wound healing. This study used an impedance sensor to monitor the effective resistance and permittivity of artificial tissues, alginate hydrogel with encapsulated fibroblasts, which were kept viable through the use of a bespoke microfluidic system. The observed transient impedance responses upon the application of identical compressive normal loads differed between acellular hydrogels and hydrogels in which fibroblasts were encapsulated. These differences resulted from changes in the conductivity and permeability of the hydrogel due to the presence of the encapsulated fibroblasts, and transient changes in ion concentrations due to mechanotransduction effects.</p></abstract>

Mechanotransduction is of fundamental importance in cell physiology, facilitating sensing in touch and hearing as well as tissue development and wound healing. This study used an impedance sensor to monitor the effective resistance and permittivity of artificial tissues, alginate hydrogel with encapsulated fibroblasts, which were kept viable through the use of a bespoke microfluidic system. The observed transient impedance responses upon the application of identical compressive normal loads differed between acellular hydrogels and hydrogels in which fibroblasts were encapsulated. These differences resulted from changes in the conductivity and permeability of the hydrogel due to the presence of the encapsulated fibroblasts, and transient changes in ion concentrations due to mechanotransduction effects.

Transient bioimpedance monitoring of mechanotransduction in artificial tissue during indentation

Journal of Electrical Bioimpedance

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mechanotransduction is of fundamental importance in cell physiology, facilitating sensing in touch and hearing as well as tissue development and wound healing....

Transient bioimpedance monitoring of mechanotransduction in artificial tissue during indentation

Article Category: Articles

Pubblicato online: 09 set 2014

Pagine: 55 - 73

Ricevuto: 29 apr 2014

DOI: https://doi.org/10.5617/jeb.869

Parole chiave
Bioimpedance, mechanotransduction, restitution, cells, artificial skin, tissue engineering

© 2014 David Cheneler, James Bowen, Georgia Kaklamani, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Transient bioimpedance monitoring of mechanotransduction in artificial tissue during indentation

Article Category: Articles

Pubblicato online: 09 set 2014

Pagine: 55 - 73

Ricevuto: 29 apr 2014

DOI: https://doi.org/10.5617/jeb.869

Parole chiaveBioimpedance, mechanotransduction, restitution, cells, artificial skin, tissue engineering

© 2014 David Cheneler, James Bowen, Georgia Kaklamani, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
Bioimpedance, mechanotransduction, restitution, cells, artificial skin, tissue engineering