<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> In the study, a 122 bp fragment of <italic>gag </italic>gene encoding immunodominant epitope on capsid protein of small ruminant lentiviruses (SRLVs) found in sheep was amplified by PCR and analysed by SSCP and sequencing. Out of 30 DNA samples, five showed different migration patterns, demonstrating the individual variations within <italic>gag </italic>sequences, which were confirmed afterwards by sequence analysis. In two samples nucleotide changes yielded amino acid substitutions highlighting the conservative nature of gag encoded immunoreactive epitope but also potencial insensitivity of a single-strain-based immunoassay.</p>
</abstract>

In the study, a 122 bp fragment of gag gene encoding immunodominant epitope on capsid protein of small ruminant lentiviruses (SRLVs) found in sheep was amplified by PCR and analysed by SSCP and sequencing. Out of 30 DNA samples, five showed different migration patterns, demonstrating the individual variations within gag sequences, which were confirmed afterwards by sequence analysis. In two samples nucleotide changes yielded amino acid substitutions highlighting the conservative nature of gag encoded immunoreactive epitope but also potencial insensitivity of a single-strain-based immunoassay.

Diversity of GAG Gene Sequence Encoding Immunodominant Epitope on Capsid Protein of Lentiviruses from Sheep in Poland

Journal of Veterinary Research

In the study, a 122 bp fragment of gag gene encoding immunodominant epitope on capsid protein of small ruminant lentiviruses (SRLVs) found in sheep was...

Diversity of GAG Gene Sequence Encoding Immunodominant Epitope on Capsid Protein of Lentiviruses from Sheep in Poland

Pubblicato online: 17 gen 2013

Pagine: 431 - 434

DOI: https://doi.org/10.2478/v10213-012-0076-3

Parole chiave
small ruminant lentiviruses, SSCP, gag gene sequence, Poland

This content is open access.

Diversity of GAG Gene Sequence Encoding Immunodominant Epitope on Capsid Protein of Lentiviruses from Sheep in Poland

Pubblicato online: 17 gen 2013

Pagine: 431 - 434

DOI: https://doi.org/10.2478/v10213-012-0076-3

Parole chiavesmall ruminant lentiviruses, SSCP, gag gene sequence, Poland

This content is open access.

Parole chiave
small ruminant lentiviruses, SSCP, gag gene sequence, Poland