<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Wood constitutes the unique source of DNA in dead trees, but extraction of adequate quality DNA from dry wood is usually challenging. However, many different molecular studies require the use of such DNA. We have standardized and validated a modified CTAB protocol to isolate DNA from dry wood from <italic>Abies pinsapo</italic> and <italic>Cedrus atlantica</italic> species. Due to the degradation and very little DNA that is normally present in the wood from dead trees we have developed a PCR based test to certify the quality of the extracted samples. In the present study, we have proved too the effectiveness of this methodology to isolate DNA from conifer dry wood samples of sufficient quality to perform further molecular genetic experiments.</p></abstract>

Wood constitutes the unique source of DNA in dead trees, but extraction of adequate quality DNA from dry wood is usually challenging. However, many different molecular studies require the use of such DNA. We have standardized and validated a modified CTAB protocol to isolate DNA from dry wood from Abies pinsapo and Cedrus atlantica species. Due to the degradation and very little DNA that is normally present in the wood from dead trees we have developed a PCR based test to certify the quality of the extracted samples. In the present study, we have proved too the effectiveness of this methodology to isolate DNA from conifer dry wood samples of sufficient quality to perform further molecular genetic experiments.

DNA extraction and amplification from Pinaceae dry wood

Departamento de Genética, Fisiología y Microbiología, Unidad de Genética, Facultad de Biología

Departamento de Sistemas Físicos, Químicos y Naturales

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"DNA extraction and amplification from Pinaceae dry wood"}

Wood constitutes the unique source of DNA in dead trees, but extraction of adequate quality DNA from dry wood is usually challenging. However, many different...

DNA extraction and amplification from Pinaceae dry wood

Pubblicato online: 15 giu 2019

Pagine: 55 - 57

DOI: https://doi.org/10.2478/sg-2019-0010

Parole chiave
dry wood, DNA isolation, CTAB, 18S, Abies pinsapo, Cedrus atlantica

© 2019 Belén Méndez-Cea et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

DNA extraction and amplification from Pinaceae dry wood

Pubblicato online: 15 giu 2019

Pagine: 55 - 57

DOI: https://doi.org/10.2478/sg-2019-0010

Parole chiavedry wood, DNA isolation, CTAB, 18S, Abies pinsapo, Cedrus atlantica

© 2019 Belén Méndez-Cea et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
dry wood, DNA isolation, CTAB, 18S, Abies pinsapo, Cedrus atlantica