<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>The aim of the present study was to compare the effectiveness of the concentration techniques of flotation-centrifugation with zinc chloride (FZn) (δ = 1.45) with the spontaneous sedimentation (SSed) and the sucrose flotation-centrifugation (FSuc) (δ = 1.2) to recuperate gastrointestinal parasites from camelid fecal samples. The technique with more positive results for the detection of Nematodirus sp., Trichuris sp., strongyle-type eggs and Eimeria macusaniensis oocysts was the FZn. For Trichuris sp. and Eimeria macusaniensis, the higher coverglass counts were detected by FZn procedure. No significant differences were registered among centrifugation flotation techniques for Nematodirus spp. Coverglass count for strongyle-type eggs was significantly higher for FSuc than FZn (p = 0.0005) or SSed (p = 0.0005), being also significantly higher for FZn than for SSed (p = 0.008). FZn is a sensitive technique that allows the recovery of parasite elements with high density and it exerts low osmotic pressure avoiding parasite deformation.</p></abstract>

The aim of the present study was to compare the effectiveness of the concentration techniques of flotation-centrifugation with zinc chloride (FZn) (δ = 1.45) with the spontaneous sedimentation (SSed) and the sucrose flotation-centrifugation (FSuc) (δ = 1.2) to recuperate gastrointestinal parasites from camelid fecal samples. The technique with more positive results for the detection of Nematodirus sp., Trichuris sp., strongyle-type eggs and Eimeria macusaniensis oocysts was the FZn. For Trichuris sp. and Eimeria macusaniensis, the higher coverglass counts were detected by FZn procedure. No significant differences were registered among centrifugation flotation techniques for Nematodirus spp. Coverglass count for strongyle-type eggs was significantly higher for FSuc than FZn (p = 0.0005) or SSed (p = 0.0005), being also significantly higher for FZn than for SSed (p = 0.008). FZn is a sensitive technique that allows the recovery of parasite elements with high density and it exerts low osmotic pressure avoiding parasite deformation.

Effectiveness of coproscopic concentration techniques

Laboratorio de Paleoparasitología y Arqueología Contextual, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3350, 7600, Mar del Plata, Buenos Aires, Argentina

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Effectiveness of coproscopic concentration techniques"}

The aim of the present study was to compare the effectiveness of the concentration techniques of flotation-centrifugation with zinc chloride (FZn) (δ = 1.45)...

Effectiveness of coproscopic concentration techniques

Pubblicato online: 17 set 2014

Pagine: 210 - 214

DOI: https://doi.org/10.2478/s11687-014-0231-x

Parole chiave
Concentration techniques, zinc chloride, parasite, camelid

© 2014 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effectiveness of coproscopic concentration techniques

Pubblicato online: 17 set 2014

Pagine: 210 - 214

DOI: https://doi.org/10.2478/s11687-014-0231-x

Parole chiaveConcentration techniques, zinc chloride, parasite, camelid

© 2014 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
Concentration techniques, zinc chloride, parasite, camelid