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Evaluation of Achillea millefolium essential oil compounds and biological effects on cervix cancer HeLa cell line

Mustafa Burak Acar
Mustafa Burak Acar
, 
Ebru Karadaş İbiş
Ebru Karadaş İbiş
, 
Ahmet Şimşek
Ahmet Şimşek
, 
Cem Vural
Cem Vural
, 
Coşkun Tez
Coşkun Tez
 e 
Servet Özcan
Servet Özcan
   | 18 gen 2020
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Article Category: Research article
Pubblicato online: 18 gen 2020
Pagine: 17 - 24
DOI: https://doi.org/10.2478/ebtj-2020-0003
Parole chiave
© 2020 Mustafa Burak Acar, Ebru Karadaş İbiş, Ahmet Şimşek, Cem Vural, Coşkun Tez, and Servet Özcan, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Figure 1

Ingenuity Pathway Analysis software for the network analysis for the most abundant four molecules (1,8-Cineole, Camphor, β-Eudesmol and Camphene). Compound related networks were depicted in A, B, C and D, respectively.
Ingenuity Pathway Analysis software for the network analysis for the most abundant four molecules (1,8-Cineole, Camphor, β-Eudesmol and Camphene). Compound related networks were depicted in A, B, C and D, respectively.

Figure 2

Annexin V / 7-AAD test results total apoptosis rates for HeLa cell line. In comparison with DMSO control group essential oil shows apoptotic effects on cancer cell line.
Annexin V / 7-AAD test results total apoptosis rates for HeLa cell line. In comparison with DMSO control group essential oil shows apoptotic effects on cancer cell line.

Figure 3

Yarrow essential oils treatment results as Dot-Plot on HeLa cell line. Number of healthy cells were decreased after essential oil treatment and apoptotic and dead cell percentage were increased.
Yarrow essential oils treatment results as Dot-Plot on HeLa cell line. Number of healthy cells were decreased after essential oil treatment and apoptotic and dead cell percentage were increased.

Figure 4

Annexin V / 7-AAD test and live cell percentage of HeLa cells. Number of living cells were decreased significantly when compared DMSO control group.
Annexin V / 7-AAD test and live cell percentage of HeLa cells. Number of living cells were decreased significantly when compared DMSO control group.

Figure 5

Cell cycle test for HeLa cell line. Essential oil application blocks the cell cycle in G0/G1 phase when compared DMSO control group.
Cell cycle test for HeLa cell line. Essential oil application blocks the cell cycle in G0/G1 phase when compared DMSO control group.

Figure 6

Ki-67 test and proliferating cell (+) ratios for HeLa cell line. Ki-67 proliferation test shows in correlation with cell cycle tests essential oil application inhibits the proliferation of cervix cancer cells in all concentration.
Ki-67 test and proliferating cell (+) ratios for HeLa cell line. Ki-67 proliferation test shows in correlation with cell cycle tests essential oil application inhibits the proliferation of cervix cancer cells in all concentration.

Figure 7

Proliferating cell percentage as Volcano Plot graph of HeLa cells in Control, DMSO Control and ACH groups. According to Ki-67 Proliferation Assay results proliferation of cells blocked by essential oil of A. millefolium.
Proliferating cell percentage as Volcano Plot graph of HeLa cells in Control, DMSO Control and ACH groups. According to Ki-67 Proliferation Assay results proliferation of cells blocked by essential oil of A. millefolium.

GC-MS analysis of essential oil components from A. millefolium. According to Peak report 10 different molecule were identified in essential oil. Four of these molecules are significantly higher than other molecules.

Peak Report Total Ion Chromatogram
Peak #MoleculeR. TimeAreaArea % ↓
31,8-Cineole8.1278332877127.34
4Camphor (CAS)9.5857401701624.28
10.beta.-Eudesmol (CAS)14.1585712936418.74
8Caryophyllene oxide13.595253497288.32
5Bicyclo[2.2.1]heptan-2-ol. 1.7.7-trimethyl-, (1S-endo)-9.832163136775.35
1Camphene7.053109549913.59
9Viridiflorol13.681106564583.50
2Bicyclo[3.1.1]heptan. 6.6-dimethyl-2-methylene-. (1S)-7.42499194903.25
7.alpha.-Terpineol10.00586024162.82
63-Cyclohexen-1-ol. 4-methyl-1-(1-methylethyl)-. (R)-9.88085282332.80
304800144100.00
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Inglese
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