<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Red yeast <italic>Cystofilobasidium capitatum</italic> autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells. Yeasts were cultivated in 2L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and HPLC determination of pigments. To compare FLIM (Fluorescence Lifetime Imaging Microscopy) images and coupled data (obtained by CLSM) with model systems, FLIM analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, and with constant addition of beta-carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoid protective system in membranes of different compartments of yeast cell, especially cytoplasmic membrane.</p></abstract>

Red yeast Cystofilobasidium capitatum autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells. Yeasts were cultivated in 2L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and HPLC determination of pigments. To compare FLIM (Fluorescence Lifetime Imaging Microscopy) images and coupled data (obtained by CLSM) with model systems, FLIM analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, and with constant addition of beta-carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoid protective system in membranes of different compartments of yeast cell, especially cytoplasmic membrane.

Fluorescence lifetime imaging of red yeast <italic>Cystofilobasidium capitatum</italic> during growth

Materials Research Centre, Faculty of Chemistry

The EuroBiotech Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fluorescence lifetime imaging of red yeast  during growth

Red yeast Cystofilobasidium capitatum autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids...

Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth

Article Category: Research Article

Pubblicato online: 25 apr 2018

Pagine: 114 - 120

DOI: https://doi.org/10.2478/ebtj-2018-0015

Parole chiave
autofluorescence, carotenoids, fluorescence lifetime imaging, red yeasts

© 2018 Martin Vanek, Filip Mravec, Martin Szotkowski, Dana Byrtusova, Andrea Haronikova, Milan Certik, Volha Shapaval, Ivana Marova, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth

Article Category: Research Article

Pubblicato online: 25 apr 2018

Pagine: 114 - 120

DOI: https://doi.org/10.2478/ebtj-2018-0015

Parole chiaveautofluorescence, carotenoids, fluorescence lifetime imaging, red yeasts

© 2018 Martin Vanek, Filip Mravec, Martin Szotkowski, Dana Byrtusova, Andrea Haronikova, Milan Certik, Volha Shapaval, Ivana Marova, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
autofluorescence, carotenoids, fluorescence lifetime imaging, red yeasts