<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Granulosa cells (GCs) are a major component found in ovarian follicular fluid among oocytes, theca cells, and ovarian surface epithelial (OSE) cells. GCs are steroidogenic and have morphological functions that are important for the development of the follicular follicle. The follicle protects the developing female egg. GCs are also essential for the maturation of the female germ cell. Stem cell properties have been found in luteinised GCs and in vivo cultures have the potential to differentiate to become cells found outside of the ovary. Both three-dimensional (3D) culturing and mouse embryonic fibroblast (MEF) medium have been used to help improve the culturing lifespan of GCs so that their profound proliferation and differentiation capabilities can be studied. Small RNAs called MicroRNAs (miRNAs) are released from exosomes originating from GCs, and they are involved in transforming growth factor (TGF)-β signalling, follicle-stimulating hormone, hormone-related miRNAs, and apoptosis-related pathway. Finding the miRNAs involved in these pathways, and the mechanisms controlling GCs are important to treating conditions like polycystic ovary syndrome (PCOS), and creating new drug therapies. Besides GCs, ovarian stem cells (OSCs) were discovered in the OSE, and they are believed to be derived from very small embryonic stem cells (VSELs). Transplanting blood mononuclear cells, cell Hormone Therapy (cHT) with bone marrow stem cell supplement and increasing vitamin C levels are all therapies currently being researched into that involve GCs to combat the effects of aging and infertility.</p><p><bold>Running title</bold>: Stemness of ovarian follicular cells</p></abstract>

Granulosa cells (GCs) are a major component found in ovarian follicular fluid among oocytes, theca cells, and ovarian surface epithelial (OSE) cells. GCs are steroidogenic and have morphological functions that are important for the development of the follicular follicle. The follicle protects the developing female egg. GCs are also essential for the maturation of the female germ cell. Stem cell properties have been found in luteinised GCs and in vivo cultures have the potential to differentiate to become cells found outside of the ovary. Both three-dimensional (3D) culturing and mouse embryonic fibroblast (MEF) medium have been used to help improve the culturing lifespan of GCs so that their profound proliferation and differentiation capabilities can be studied. Small RNAs called MicroRNAs (miRNAs) are released from exosomes originating from GCs, and they are involved in transforming growth factor (TGF)-β signalling, follicle-stimulating hormone, hormone-related miRNAs, and apoptosis-related pathway. Finding the miRNAs involved in these pathways, and the mechanisms controlling GCs are important to treating conditions like polycystic ovary syndrome (PCOS), and creating new drug therapies. Besides GCs, ovarian stem cells (OSCs) were discovered in the OSE, and they are believed to be derived from very small embryonic stem cells (VSELs). Transplanting blood mononuclear cells, cell Hormone Therapy (cHT) with bone marrow stem cell supplement and increasing vitamin C levels are all therapies currently being researched into that involve GCs to combat the effects of aging and infertility.Running title: Stemness of ovarian follicular cells

Ovarian follicular cells - living in the shadow of stemness cellular competence

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Ovarian follicular cells - living in the shadow of stemness cellular competence"}

Granulosa cells (GCs) are a major component found in ovarian follicular fluid among oocytes, theca cells, and ovarian surface epithelial (OSE) cells. GCs are...

Ovarian follicular cells - living in the shadow of stemness cellular competence

Pubblicato online: 08 nov 2019

Pagine: 134 - 140

Ricevuto: 21 set 2019

Accettato: 29 ott 2019

DOI: https://doi.org/10.2478/acb-2019-0018

Parole chiave
microRNAs, ovarian stem cells, infertility treatments

© 2019 Lisa Moncrieff, Paul Mozdziak, Michal Jeseta, Marie Machatkova, Wiesława Kranc, Bartosz Kempisty, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Ovarian follicular cells - living in the shadow of stemness cellular competence

Pubblicato online: 08 nov 2019

Pagine: 134 - 140

Ricevuto: 21 set 2019

Accettato: 29 ott 2019

DOI: https://doi.org/10.2478/acb-2019-0018

Parole chiavemicroRNAs, ovarian stem cells, infertility treatments

© 2019 Lisa Moncrieff, Paul Mozdziak, Michal Jeseta, Marie Machatkova, Wiesława Kranc, Bartosz Kempisty, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
microRNAs, ovarian stem cells, infertility treatments