<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Understanding the genetic diversity of <italic>Acacia melanoxylon</italic> is very important in species selection and improvement. The present study aimed to identify microsatellite markers and determine the genetic diversity of 45 preferred clones selected from 9 Chinese districts. Seventy-six simple sequence repeat (SSR) markers developed for other <italic>Acacia</italic> species were screened. Seventeen SSR markers showed polymorphic patterns and amplified 134 alleles. Polymorphism information content (PIC) values ranged from 0.044 to 0.911. The average allele number per locus ranged between 2 and 18, averaging 8.06. Nine SSR markers were highly informative, with PIC values above 0.60. These findings demonstrated that SSR markers could be used to differentiate <italic>A. melanoxylon</italic> genotypes. Cluster analysis using UPGMA separated the 45 clones into 3 distinct groups at a similarity coefficient of 0.72. The clone groups identified in this study would be useful in developing intraspecific hybrids to exploit hybrid vigor as well as for commercial cultivation and genetic base broadening. The DNA fingerprints obtained for each clone could be used for biodiversity conservation.</p></abstract>

Understanding the genetic diversity of Acacia melanoxylon is very important in species selection and improvement. The present study aimed to identify microsatellite markers and determine the genetic diversity of 45 preferred clones selected from 9 Chinese districts. Seventy-six simple sequence repeat (SSR) markers developed for other Acacia species were screened. Seventeen SSR markers showed polymorphic patterns and amplified 134 alleles. Polymorphism information content (PIC) values ranged from 0.044 to 0.911. The average allele number per locus ranged between 2 and 18, averaging 8.06. Nine SSR markers were highly informative, with PIC values above 0.60. These findings demonstrated that SSR markers could be used to differentiate A. melanoxylon genotypes. Cluster analysis using UPGMA separated the 45 clones into 3 distinct groups at a similarity coefficient of 0.72. The clone groups identified in this study would be useful in developing intraspecific hybrids to exploit hybrid vigor as well as for commercial cultivation and genetic base broadening. The DNA fingerprints obtained for each clone could be used for biodiversity conservation.

Development of simple sequence repeat (SSR) markers and genetic diversity analysis in blackwood (<italic>Acacia melanoxylon</italic>) clones in china

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Development of simple sequence repeat (SSR) markers and genetic diversity analysis in blackwood () clones in china

Understanding the genetic diversity of Acacia melanoxylon is very important in species selection and improvement. The present study aimed to identify...

Development of simple sequence repeat (SSR) markers and genetic diversity analysis in blackwood (Acacia melanoxylon) clones in china

Pubblicato online: 12 giu 2017

Pagine: 49 - 54

DOI: https://doi.org/10.1515/sg-2016-0006

Parole chiave
Acacia melanoxylon, SSR, genetic diversity, polymorphism information content

© 2016 Chunjie Fan et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Development of simple sequence repeat (SSR) markers and genetic diversity analysis in blackwood (Acacia melanoxylon) clones in china

Pubblicato online: 12 giu 2017

Pagine: 49 - 54

DOI: https://doi.org/10.1515/sg-2016-0006

Parole chiaveAcacia melanoxylon, SSR, genetic diversity, polymorphism information content

© 2016 Chunjie Fan et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
Acacia melanoxylon, SSR, genetic diversity, polymorphism information content