<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Induction of 2n pollen is a required technique for cultivating polyploid via sexual polyploidy. Orthogonal design or Taguchi Design was applied to select the best treatment process of 2n pollen induction in <italic>Populus</italic> × <italic>popularis</italic> from different levels of the meiosis stage of male flower buds, colchicine concentration, times of injection, and interval between injections. Flow cytometry and chromosome counting were used to identify the triploids from the offspring of <italic>P.</italic> × <italic>euramericana</italic>. (Dode) Guinier pollinated with induced pollen of <italic>P.</italic> × <italic>popularis</italic>. The results showed that high 2n pollen rate can be achieved by selecting the flower buds during diakinesis stage in meiosis, and then injecting 0.6% colchicine 4 times with 2 hours interval. The 2n pollen rate reached 62.10% by this process, and two triploids were obtained, which indicates that it is possible for cultivating triploids via 2n pollen induction by colchicine treatment in poplar. Results and protocol related to 2n pollen induction, polyploid identification and effect of 2n pollen in this study might be applicable in polyploidy breeding in section <italic>Aigeiros</italic> and <italic>Tacamahaca</italic> of poplar.</p></abstract>

Induction of 2n pollen is a required technique for cultivating polyploid via sexual polyploidy. Orthogonal design or Taguchi Design was applied to select the best treatment process of 2n pollen induction in Populus × popularis from different levels of the meiosis stage of male flower buds, colchicine concentration, times of injection, and interval between injections. Flow cytometry and chromosome counting were used to identify the triploids from the offspring of P. × euramericana. (Dode) Guinier pollinated with induced pollen of P. × popularis. The results showed that high 2n pollen rate can be achieved by selecting the flower buds during diakinesis stage in meiosis, and then injecting 0.6% colchicine 4 times with 2 hours interval. The 2n pollen rate reached 62.10% by this process, and two triploids were obtained, which indicates that it is possible for cultivating triploids via 2n pollen induction by colchicine treatment in poplar. Results and protocol related to 2n pollen induction, polyploid identification and effect of 2n pollen in this study might be applicable in polyploidy breeding in section Aigeiros and Tacamahaca of poplar.

Induction of 2n pollen by colchicine in <italic>Populus</italic> × <italic>popularis</italic> and its triploids breeding

National Engineering Laboratory for Tree Breeding

Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education

The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration

Department of Forestry and Environmental Resources

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Induction of 2n pollen by colchicine in  ×  and its triploids breeding

Induction of 2n pollen is a required technique for cultivating polyploid via sexual polyploidy. Orthogonal design or Taguchi Design was applied to select the...

Induction of 2n pollen by colchicine in Populus × popularis and its triploids breeding

Pubblicato online: 05 ago 2017

Pagine: 155 - 160

Ricevuto: 22 nov 2010

DOI: https://doi.org/10.1515/sg-2011-0021

Parole chiave
2n pollen, poplar, polyploid breeding, colchicine, Orthogonal design

© 2011 X. J. Xi et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Induction of 2n pollen by colchicine in Populus × popularis and its triploids breeding

Pubblicato online: 05 ago 2017

Pagine: 155 - 160

Ricevuto: 22 nov 2010

DOI: https://doi.org/10.1515/sg-2011-0021

Parole chiave2n pollen, poplar, polyploid breeding, colchicine, Orthogonal design

© 2011 X. J. Xi et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
2n pollen, poplar, polyploid breeding, colchicine, Orthogonal design