<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Nothofagus is the main component of southern South American temperate forests. Overexploitation in the past has led to the loss of 40% of the original distribution range. Genetic diversity as well as biological processes shaping the distribution of the genetic variation (e.g. gene flow) constitutes basic knowledge for the implementation of conservation measures and for the definition of Evolutionary Significant Units. Nuclear microsatellites are the marker of choice for gene flow and fine-scale genetic structure studies. We enlarged a previous set of microsatellites (SSRs) for South American Nothofagus species, with special concern to Nothofagus nervosa (Phil.) Dim. et Mil. Five new SSRs are presented with allele numbers up to 12 in a single population. The primers transferred well to five related species (N. obliqua (Mirb.) Oerst, N. glauca (Phil.) Krasser, N. dombeyi (Mirb.) Oerst , N. pumilio (Poepp et Endl.) Krasser and N. antarctica (G. Forster) Oerst, with allele numbers up to 11. The high level of polymorphism promises a sufficient power for gene flow and parentage analyses.</p></abstract>

Nothofagus is the main component of southern South American temperate forests. Overexploitation in the past has led to the loss of 40% of the original distribution range. Genetic diversity as well as biological processes shaping the distribution of the genetic variation (e.g. gene flow) constitutes basic knowledge for the implementation of conservation measures and for the definition of Evolutionary Significant Units. Nuclear microsatellites are the marker of choice for gene flow and fine-scale genetic structure studies. We enlarged a previous set of microsatellites (SSRs) for South American Nothofagus species, with special concern to Nothofagus nervosa (Phil.) Dim. et Mil. Five new SSRs are presented with allele numbers up to 12 in a single population. The primers transferred well to five related species (N. obliqua (Mirb.) Oerst, N. glauca (Phil.) Krasser, N. dombeyi (Mirb.) Oerst , N. pumilio (Poepp et Endl.) Krasser and N. antarctica (G. Forster) Oerst, with allele numbers up to 11. The high level of polymorphism promises a sufficient power for gene flow and parentage analyses.

Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Nothofagus is the main component of southern South American temperate forests. Overexploitation in the past has led to the loss of 40% of the original...

Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species

Pubblicato online: 14 ott 2017

Pagine: 82 - 85

Ricevuto: 13 mar 2007

DOI: https://doi.org/10.1515/sg-2008-0013

Parole chiave
Nothofagus, microsatellites, South America, gene flow, fine-scale genetic structure

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species

Pubblicato online: 14 ott 2017

Pagine: 82 - 85

Ricevuto: 13 mar 2007

DOI: https://doi.org/10.1515/sg-2008-0013

Parole chiaveNothofagus, microsatellites, South America, gene flow, fine-scale genetic structure

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
Nothofagus, microsatellites, South America, gene flow, fine-scale genetic structure