<abstract xmlns="http://www.w3.org/1999/xhtml">The novel WBN/Kob-<italic>Leprfa</italic> (<italic>fa/fa</italic>) congenic rat strain is considered a useful rat model of type 2 diabetes mellitus (T2DM). Accumulating findings suggest that low-grade inflammation is a causative factor in T2DM and that circulating levels of inflammatory cytokines are associated with insulin resistance. However, inflammatory cytokine profiles and their correlations with T2DM development/progression in <italic>fa/fa</italic> rats have not been studied. In this study, we found that the <italic>fa/fa</italic> rats had considerably high plasma levels of interleukin (IL)-1α. Abundant cecal IL-1α mRNA expression and cecal inflammation with infiltrating IL-1α-producing macrophages was observed in <italic>fa/fa</italic> rats. Bone marrow derived macrophages from <italic>fa/fa</italic> rats expressed high levels of IL-1α upon lipopolysaccharide stimulation. Furthermore, <italic>Syphacia muris</italic> infection, which delays the onset of T2DM, reduced both plasma and cecal IL-1α levels in <italic>fa/fa</italic> rats. These results suggest that macrophage infiltration and IL-1α secretion comprise an important part of T2DM development and that <italic>S. muris</italic> infection inhibits pro-inflammatory cytokine expression in <italic>fa/fa</italic> rats.</abstract>

The novel WBN/Kob-Leprfa (fa/fa) congenic rat strain is considered a useful rat model of type 2 diabetes mellitus (T2DM). Accumulating findings suggest that low-grade inflammation is a causative factor in T2DM and that circulating levels of inflammatory cytokines are associated with insulin resistance. However, inflammatory cytokine profiles and their correlations with T2DM development/progression in fa/fa rats have not been studied. In this study, we found that the fa/fa rats had considerably high plasma levels of interleukin (IL)-1α. Abundant cecal IL-1α mRNA expression and cecal inflammation with infiltrating IL-1α-producing macrophages was observed in fa/fa rats. Bone marrow derived macrophages from fa/fa rats expressed high levels of IL-1α upon lipopolysaccharide stimulation. Furthermore, Syphacia muris infection, which delays the onset of T2DM, reduced both plasma and cecal IL-1α levels in fa/fa rats. These results suggest that macrophage infiltration and IL-1α secretion comprise an important part of T2DM development and that S. muris infection inhibits pro-inflammatory cytokine expression in fa/fa rats.

High IL-1α production was induced in the WBN/Kob-<italic>Leprfa</italic> type 2 diabetes mellitus rat model and inhibited by <italic>Syphacia muris</italic> infection

Department of Veterinary Medicine, School of Veterinary Medicine

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

High IL-1α production was induced in the WBN/Kob- type 2 diabetes mellitus rat model and inhibited by  infection

The novel WBN/Kob-Leprfa (fa/fa) congenic rat strain is considered a useful rat model of type 2 diabetes mellitus (T2DM). Accumulating findings suggest that...

High IL-1α production was induced in the WBN/Kob-Lepr^fa type 2 diabetes mellitus rat model and inhibited by Syphacia muris infection

Pubblicato online: 27 gen 2018

Pagine: 12 - 20

Ricevuto: 03 mar 2017

Accettato: 05 set 2017

DOI: https://doi.org/10.1515/helm-2017-0050

Parole chiave
IL-1α, macrophage, type 2 diabetes mellitus, WBN/Kob-

© 2018 M. Okamoto et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

High IL-1α production was induced in the WBN/Kob-Leprfa type 2 diabetes mellitus rat model and inhibited by Syphacia muris infection

Pubblicato online: 27 gen 2018

Pagine: 12 - 20

Ricevuto: 03 mar 2017

Accettato: 05 set 2017

DOI: https://doi.org/10.1515/helm-2017-0050

Parole chiaveIL-1α, macrophage, type 2 diabetes mellitus, WBN/Kob-

© 2018 M. Okamoto et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

High IL-1α production was induced in the WBN/Kob-Lepr^fa type 2 diabetes mellitus rat model and inhibited by Syphacia muris infection

Parole chiave
IL-1α, macrophage, type 2 diabetes mellitus, WBN/Kob-