<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.</p></abstract>

The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from non-vaccinated swine by polymerase chain reaction. All animals were reared in extensive livestock farming systems in different geographical districts of Republic of Srpska, Bosnia and Herzegovina. Porcine circovirus 2 DNA was detected in four lymph node and two spleen samples (15%), while porcine parvovirus DNA was identified in five lymph node samples (12.5%). The presence of both viruses was detected in three lymph node samples (7.5%). Partial nucleotide sequence of ORF1 gene of 2 porcine circovirus 2 and VP2 gene of 2 porcine parvovirus isolates was determined. The nucleotide sequences of two PCV2 isolates from RS-BIH included in phylogenetic typing are similar and cluster together with the strain Mantova isolated from domestic pigs in Italy, strains DE006-14 and DE222-13 isolated from pigs in Germany as well as with the strain Jvnan isolated from pigs in China. Also, analyzed PCV2 isolates were partially similar to the strain NIV-C SRB isolated from pigs in Serbia. The nucleotide sequences of two PPV isolates that were included in phylogenetic typing showed a high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated from pigs in USA and strains 77 and LZ isolated from pigs in China.

Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

Virology Department, Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr Subotića 8, Belgrade, Serbia

Department of Microbiology, Faculty of Veterinary Medicine University of Belgrade, Bul. Oslobođenja 18, Belgrade, Serbia

Scientific Veterinary Institute of Serbia, Vojvode Toze 14, Belgrade, Serbia

Department of Economics and Statistics Faculty of Veterinary Medicine University of Belgrade, Bul. Oslobođenja 18, Belgrade, Serbia

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The presence of porcine circovirus 2 and porcine parvovirus was examined in forty clinical samples of spleen, lymph nodes and lungs originating from...

Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

Pubblicato online: 23 mar 2016

Pagine: 51 - 60

Ricevuto: 10 set 2015

Accettato: 28 dic 2015

DOI: https://doi.org/10.1515/acve-2016-0004

Parole chiave
porcine circovirus 2, porcine parvovirus, nucleotide sequence, Republic of Srpska, Bosnia and Herzegovina

© by Bojan Lukač

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

Pubblicato online: 23 mar 2016

Pagine: 51 - 60

Ricevuto: 10 set 2015

Accettato: 28 dic 2015

DOI: https://doi.org/10.1515/acve-2016-0004

Parole chiaveporcine circovirus 2, porcine parvovirus, nucleotide sequence, Republic of Srpska, Bosnia and Herzegovina

© by Bojan Lukač

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
porcine circovirus 2, porcine parvovirus, nucleotide sequence, Republic of Srpska, Bosnia and Herzegovina