<abstract xmlns="http://www.w3.org/1999/xhtml"> This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The method was evaluated for precision, linearity, LOD, LOQ, accuracy and robustness. Chromatographic analysis of the extracts revealed the presence of three main flavonols, quercetin, quercetin-4′-O-glucoside and quercetin-3,4′-O-diglucoside in the majority of analyzed cultivars. The content of flavonols in the analyzed extracts of onion bulbs varied from 123 (‘Exihibition’) to 1079 mg kg-1 fresh mass (fm) (‘Hybing’) in edible parts, and from 1727 (‘Hyline’) to 28949 mg kg-1 fm (‘Red Baron’) in outer scales. The bulbs of two shallot cultivars contained 209 (‘Ambition’) and 523 mg kg-1 fm (‘Matador’) of flavonols in edible parts and 5426 and 8916 mg kg-1 fm in outer scales, respectively.</abstract>

This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The method was evaluated for precision, linearity, LOD, LOQ, accuracy and robustness. Chromatographic analysis of the extracts revealed the presence of three main flavonols, quercetin, quercetin-4′-O-glucoside and quercetin-3,4′-O-diglucoside in the majority of analyzed cultivars. The content of flavonols in the analyzed extracts of onion bulbs varied from 123 (‘Exihibition’) to 1079 mg kg-1 fresh mass (fm) (‘Hybing’) in edible parts, and from 1727 (‘Hyline’) to 28949 mg kg-1 fm (‘Red Baron’) in outer scales. The bulbs of two shallot cultivars contained 209 (‘Ambition’) and 523 mg kg-1 fm (‘Matador’) of flavonols in edible parts and 5426 and 8916 mg kg-1 fm in outer scales, respectively.

TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum

Department of Pharmacognosy with Medicinal Plants Garden, Medicine Medical University of Gdańsk 80-416 Gdańsk,

Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk 80-416 Gdańsk,

Department of Pharmaceutical Technology, Faculty of Pharmacy with Subfaculty of Laboratory Medicine Medical University of Gdańsk 80-416 Gdańsk,

Department of Pharmaceutical Technology Faculty of Pharmacy with Subfaculty of Laboratory Medicine Medical University of Gdańsk 80-416 Gdańsk,

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots....

TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum

Pubblicato online: 15 ott 2016

Pagine: 543 - 554

Accettato: 20 apr 2016

DOI: https://doi.org/10.1515/acph-2016-0038

Parole chiave
Allium cepa, Allium ascalonicum, cultivar varieties, flavonols, TLC, quantitative analysis

© by Miroslawa Krauze-Baranowska

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum

Pubblicato online: 15 ott 2016

Pagine: 543 - 554

Accettato: 20 apr 2016

DOI: https://doi.org/10.1515/acph-2016-0038

Parole chiaveAllium cepa, Allium ascalonicum, cultivar varieties, flavonols, TLC, quantitative analysis

© by Miroslawa Krauze-Baranowska

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
Allium cepa, Allium ascalonicum, cultivar varieties, flavonols, TLC, quantitative analysis