Urinary exosomes from a mouse model of chronic tubulointerstitial kidney disease induced by chronic renal ischemia–reperfusion injury and nephrectomy
Article Category: Brief communication (Original)
Publié en ligne: 31 mars 2017
Pages: 447 - 454
DOI: https://doi.org/10.5372/1905-7415.1005.507
Mots clés
© 2016 Asada Leelahavanichkul, Wiwat Chancharoenthana, Somchai Eiam-Ong
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Background
Biomarkers for the early diagnosis of chronic kidney disease (CKD) will lead to its better management. Rodent models will be helpful for biomarker discovery. Urinary exosomes, membrane bound vesicles, may contain interesting biomarkers.
Objective
To produce a mouse model of CKD with sufficient urine volume for exosome studies.
Methods
We characterized a mouse models of CKD induced by 55 min ischemia–reperfusion injury and contralateral nephrectomy at 1 wk after the chronic ischemia–reperfusion injury (Chr IR) in 7 mice and other 7 sham-operated control mice. We collected 24 h urine for urine exosome extraction.
Results
Blood urea nitrogen, serum creatinine, and proteinuria increased as early as 2 wk, and reciprocally lower hematocrit. The renal injury, proteinuria, and anemia persisted 12 wk after nephrectomy as consistent with uremic symptoms. The mouse model of CKD showed renal histopathology of interstitial fibrosis with mild glomerular injury, and failure to grow. At 12 wk, the volume of urine collected in 24 h was sufficient for exosome extraction. Urinary exosomes were demonstrated by western blots showing tumor susceptibility gene 101 protein.
Conclusions
Mice with renal injury induced by Chr IR showed renal injury and proteinuria as early as 2 wk with uremic symptoms and tubulointerstitial fibrosis. Most importantly, the 24 h volume of urine at 12 wk was sufficient for exosome extraction.