<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Ficolins are lectins that have been demonstrated to play an important role in innate immune response in a variety of diseases. Mycobacterium tuberculosis (M.tb) infection can trigger a series of changes in the host. However, the role of ficolin in tuberculosis is still unclear. Objective: We investigated the expression of ficolin in peripheral blood mononuclear cell (PBMC) in TB patients and healthy control. Methods: Using semi-quantitative RT-PCR, western blotting, and flow cytometry, we compared the expression of M-ficolin, L-ficolin, and H-ficolin in the peripheral blood mononuclear cell (PBMC), purified monocytes, and cultured dendritic cells of TB patients with healthy volunteers as controls. Results: M-ficolin expression in PBMC was significantly lower at both mRNA and protein levels in TB patients as compared to healthy controls. The lower M-ficolin level in TB patient PBMCs may be attributed to its lower level in monocytes. The expression levels of H-ficolin and L-ficolin in both healthy controls and TB patients were very low and they had no significant differences between the two groups. Conclusions: Compared to healthy controls, M-ficolin expression is significantly lower in TB patients. Measurement of M-ficolin may be a potential auxiliary tool to diagnose TB infection.</abstract>

Background: Ficolins are lectins that have been demonstrated to play an important role in innate immune response in a variety of diseases. Mycobacterium tuberculosis (M.tb) infection can trigger a series of changes in the host. However, the role of ficolin in tuberculosis is still unclear. Objective: We investigated the expression of ficolin in peripheral blood mononuclear cell (PBMC) in TB patients and healthy control. Methods: Using semi-quantitative RT-PCR, western blotting, and flow cytometry, we compared the expression of M-ficolin, L-ficolin, and H-ficolin in the peripheral blood mononuclear cell (PBMC), purified monocytes, and cultured dendritic cells of TB patients with healthy volunteers as controls. Results: M-ficolin expression in PBMC was significantly lower at both mRNA and protein levels in TB patients as compared to healthy controls. The lower M-ficolin level in TB patient PBMCs may be attributed to its lower level in monocytes. The expression levels of H-ficolin and L-ficolin in both healthy controls and TB patients were very low and they had no significant differences between the two groups. Conclusions: Compared to healthy controls, M-ficolin expression is significantly lower in TB patients. Measurement of M-ficolin may be a potential auxiliary tool to diagnose TB infection.

Expression of M-ficolin, H-ficolin, and L-ficolin in peripheral blood mononuclear cell of tuberculosis patients

Clinical laboratory of Hubei Cancer Hospital, Wuhan 434000,

The Public Health Office of Lin Qu, Shan Dong 262600, Wuhan 430060,

Department of Thoracic Cardiovascular Surgery, People’s Hospital, Wuhan University, Wuhan 430060,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Ficolins are lectins that have been demonstrated to play an important role in innate immune response in a variety of diseases. Mycobacterium...

Expression of M-ficolin, H-ficolin, and L-ficolin in peripheral blood mononuclear cell of tuberculosis patients

Article Category: Original article

Publié en ligne: 04 févr. 2017

Pages: 747 - 754

DOI: https://doi.org/10.5372/1905-7415.0506.101

Mots clés
Ficolin, monocyte, mycobacterium tuberculosis, peripheral blood mononuclear cell

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Expression of M-ficolin, H-ficolin, and L-ficolin in peripheral blood mononuclear cell of tuberculosis patients

Article Category: Original article

Publié en ligne: 04 févr. 2017

Pages: 747 - 754

DOI: https://doi.org/10.5372/1905-7415.0506.101

Mots clésFicolin, monocyte, mycobacterium tuberculosis, peripheral blood mononuclear cell

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
Ficolin, monocyte, mycobacterium tuberculosis, peripheral blood mononuclear cell