<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>In an attempt to identify novel and apoptosis/pathogen-regulated microRNAs (miRNAs) and small interfering RNAs, we performed a robust microarray screening of small RNA population from <italic>Vitis vinifera</italic> L. cv. Limberger cell suspension exposed to apoptosis activators (e.g. methyl jasmonate) or elicitors (botrycin and cinerein) derived from necrotrophic fungus <italic>Botrytis cinerea</italic> Pers. et Fries. Using a microarray expression profiling approach, we identified 22 miRNAs. We found that a majority of these miRNAs were predicted to target stress/defense-related genes of plants. Of the 22 <italic>V. vinifera</italic> miRNAs, 11 have sequence conservation in <italic>Arabidopsis thaliana</italic> but exhibited species-specific developmental and/or stress/defense-related expression patterns. Ten of the miRNAs are highly conserved in other plant species, suggesting that even conserved miRNAs may have different regulatory roles in various species. Our results show that these grapevine miRNAs can be also induced by various apoptosis inducers. Fifty-one potential targets were predicted to the newly identified miRNAs based on sequence complementarity. In addition to miRNAs, we identified 102 other novel endogenous small RNAs in <italic>Vitis</italic>, indicating that a large number of miRNAs and other small regulatory RNAs are encoded by the <italic>Vitis vinifera</italic> genome.</p></abstract>

In an attempt to identify novel and apoptosis/pathogen-regulated microRNAs (miRNAs) and small interfering RNAs, we performed a robust microarray screening of small RNA population from Vitis vinifera L. cv. Limberger cell suspension exposed to apoptosis activators (e.g. methyl jasmonate) or elicitors (botrycin and cinerein) derived from necrotrophic fungus Botrytis cinerea Pers. et Fries. Using a microarray expression profiling approach, we identified 22 miRNAs. We found that a majority of these miRNAs were predicted to target stress/defense-related genes of plants. Of the 22 V. vinifera miRNAs, 11 have sequence conservation in Arabidopsis thaliana but exhibited species-specific developmental and/or stress/defense-related expression patterns. Ten of the miRNAs are highly conserved in other plant species, suggesting that even conserved miRNAs may have different regulatory roles in various species. Our results show that these grapevine miRNAs can be also induced by various apoptosis inducers. Fifty-one potential targets were predicted to the newly identified miRNAs based on sequence complementarity. In addition to miRNAs, we identified 102 other novel endogenous small RNAs in Vitis, indicating that a large number of miRNAs and other small regulatory RNAs are encoded by the Vitis vinifera genome.

A Global Microarray Expression Profile of Grapevine miRNAs Isolated from Cell Suspensions Pre-Treated with Apoptosis Activators

Plant Production Research Center Piešťany

Institute of Botany, Slovak Academy of Sciences, Bratislava

Slovak University of Technology, Bratislava

Agriculture (Pol'nohospodárstvo)

In an attempt to identify novel and apoptosis/pathogen-regulated microRNAs (miRNAs) and small interfering RNAs, we performed a robust microarray screening of...

A Global Microarray Expression Profile of Grapevine miRNAs Isolated from Cell Suspensions Pre-Treated with Apoptosis Activators

Publié en ligne: 09 avr. 2012

Pages: 11 - 17

DOI: https://doi.org/10.2478/v10207-012-0002-y

Mots clés
L., small RNA, cell death, elicitor, methyl jasmonate

This content is open access.

A Global Microarray Expression Profile of Grapevine miRNAs Isolated from Cell Suspensions Pre-Treated with Apoptosis Activators

Publié en ligne: 09 avr. 2012

Pages: 11 - 17

DOI: https://doi.org/10.2478/v10207-012-0002-y

Mots clésL., small RNA, cell death, elicitor, methyl jasmonate

This content is open access.

Mots clés
L., small RNA, cell death, elicitor, methyl jasmonate