<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>The aim of this study is to develop a sensitive HPLC method for the quantitative determination of cytisine in serum and to characterize the pharmacokinetic behaviour of cytisine after oral and intravenous administration in rabbits. The pharmacokinetic behaviour of cytisine is studied in male and female New Zealand rabbits after oral and intravenous administration. Cytisine is administered orally (dose of 5 mg/kg b.w.) under fasting condition (12 hours) and intravenously (dose 1 mg/kg b.w.) in the marginal ear vein. Cytisine serum concentrations are measured using a highly selective and sensitive validated HPLC method with UV detection. Linearity of the method is in the range 12-2 400 μg/L; accuracy and precision are both within ± 10%, and the limit of detection is 4 μg/L. Selectivity and stability are also validated. Basic pharmacokinetic parameters of cytisine after single oral and intravenous administration are calculated using TOPFIT software. Pharmacokinetic analysis suggests a rapid but incomplete absorption of cytisine after oral administration.</p></abstract>

The aim of this study is to develop a sensitive HPLC method for the quantitative determination of cytisine in serum and to characterize the pharmacokinetic behaviour of cytisine after oral and intravenous administration in rabbits. The pharmacokinetic behaviour of cytisine is studied in male and female New Zealand rabbits after oral and intravenous administration. Cytisine is administered orally (dose of 5 mg/kg b.w.) under fasting condition (12 hours) and intravenously (dose 1 mg/kg b.w.) in the marginal ear vein. Cytisine serum concentrations are measured using a highly selective and sensitive validated HPLC method with UV detection. Linearity of the method is in the range 12-2 400 μg/L; accuracy and precision are both within ± 10%, and the limit of detection is 4 μg/L. Selectivity and stability are also validated. Basic pharmacokinetic parameters of cytisine after single oral and intravenous administration are calculated using TOPFIT software. Pharmacokinetic analysis suggests a rapid but incomplete absorption of cytisine after oral administration.

Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits

Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria

Central Laboratory of Therapeutic Drug Monitoring and Clinical Pharmacology, Faculty of Medicine, Alexander University Hospital, Medical University, Sofia, Bulgaria

Interdisciplinary Toxicology

The aim of this study is to develop a sensitive HPLC method for the quantitative determination of cytisine in serum and to characterize the pharmacokinetic...

Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits

Publié en ligne: 13 avr. 2010

Pages: 15 - 20

DOI: https://doi.org/10.2478/v10102-010-0003-5

Mots clés
cytisine, pharmacokinetics, rabbits, HPLC method

This content is open access.

Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits

Publié en ligne: 13 avr. 2010

Pages: 15 - 20

DOI: https://doi.org/10.2478/v10102-010-0003-5

Mots cléscytisine, pharmacokinetics, rabbits, HPLC method

This content is open access.

Mots clés
cytisine, pharmacokinetics, rabbits, HPLC method