<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>A successful gene delivery system requires efficiency and stability during storage. Stability studies are imperative for nanomedicines containing biotechnological products such as plasmids and targeting peptides. Chitosan-DNA-FAP-B nanoparticles are novel non-viral vectors for specific gene delivery to the lung epithelial cells. In this study, the storage stability of chitosan-DNA-FAP-B nanoparticles at -20, 5 and 24 °C was examined. Size, zeta potential and transfection efficiency of these nano-particles in storage were also evaluated. Stability studies showed that chitosan-DNA-FAP-B nanoparticles were stable after 1 month when stored at -20 °C and retained their initial size, zeta potential and transfection efficiency. However, their stability was not desirable at 5 and 24 °C. Based on these results, it can be concluded that chitosan-DNA-FAP-B nanoparticles can be a promising candidate for gene delivery to lung epithelial cells with good storage stability at -20 °C during 1 month.</p></abstract>

A successful gene delivery system requires efficiency and stability during storage. Stability studies are imperative for nanomedicines containing biotechnological products such as plasmids and targeting peptides. Chitosan-DNA-FAP-B nanoparticles are novel non-viral vectors for specific gene delivery to the lung epithelial cells. In this study, the storage stability of chitosan-DNA-FAP-B nanoparticles at -20, 5 and 24 °C was examined. Size, zeta potential and transfection efficiency of these nano-particles in storage were also evaluated. Stability studies showed that chitosan-DNA-FAP-B nanoparticles were stable after 1 month when stored at -20 °C and retained their initial size, zeta potential and transfection efficiency. However, their stability was not desirable at 5 and 24 °C. Based on these results, it can be concluded that chitosan-DNA-FAP-B nanoparticles can be a promising candidate for gene delivery to lung epithelial cells with good storage stability at -20 °C during 1 month.

Stability studies of chitosan-DNA-FAP-B nanoparticles for gene delivery to lung epithelial cells

Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Department of Biochemistry, Faculty of Science, Tarbiat Modares University, Tehran, Iran

Nobleceuticals Ltd., Birmingham, United Kingdom

Nanobiotechnology Department, Pasteur Institute of Iran, Tehran, Iran

Acta Pharmaceutica

A successful gene delivery system requires efficiency and stability during storage. Stability studies are imperative for nanomedicines containing...

Stability studies of chitosan-DNA-FAP-B nanoparticles for gene delivery to lung epithelial cells

Publié en ligne: 03 avr. 2012

Pages: 83 - 92

DOI: https://doi.org/10.2478/v10007-012-0008-z

Mots clés
kitozan, FAP-B, nanočestica, stabilnost, genska isporuka

This content is open access.

Stability studies of chitosan-DNA-FAP-B nanoparticles for gene delivery to lung epithelial cells

Publié en ligne: 03 avr. 2012

Pages: 83 - 92

DOI: https://doi.org/10.2478/v10007-012-0008-z

Mots cléskitozan, FAP-B, nanočestica, stabilnost, genska isporuka

This content is open access.

Mots clés
kitozan, FAP-B, nanočestica, stabilnost, genska isporuka