<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>BAliBASE is one of the most widely used benchmarks for multiple sequence alignment programs. The accuracy of alignment methods is measured by <italic>bali_score</italic>—an application provided together with the database. The standard accuracy measures are the Sum of Pairs (SP) and the Total Column (TC). We have found that, for non-core block columns, results calculated by <italic>bali_score</italic> are different from those obtained on the basis of the formal definitions of the measures. We do not claim that one of these measures is better than the other, but they are definitely different. Such a situation can be the source of confusion when alignments obtained using various methods are compared. Therefore, we propose a new nomenclature for the measures of the quality of multiple sequence alignments to distinguish which one was actually calculated. Moreover, we have found that the occurrence of a gap in some column in the first sequence of the reference alignment causes column discarding.</p></abstract>

BAliBASE is one of the most widely used benchmarks for multiple sequence alignment programs. The accuracy of alignment methods is measured by bali_score—an application provided together with the database. The standard accuracy measures are the Sum of Pairs (SP) and the Total Column (TC). We have found that, for non-core block columns, results calculated by bali_score are different from those obtained on the basis of the formal definitions of the measures. We do not claim that one of these measures is better than the other, but they are definitely different. Such a situation can be the source of confusion when alignments obtained using various methods are compared. Therefore, we propose a new nomenclature for the measures of the quality of multiple sequence alignments to distinguish which one was actually calculated. Moreover, we have found that the occurrence of a gap in some column in the first sequence of the reference alignment causes column discarding.

Some remarks on evaluating the quality of the multiple sequence alignment based on the BAliBASE benchmark

Institute of Computing Science, Poznań University of Technology, Piotrowo 2, 60-965 Poznań, Poland

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-714, Poznań, Poland

International Journal of Applied Mathematics and Computer Science

BAliBASE is one of the most widely used benchmarks for multiple sequence alignment programs. The accuracy of alignment methods is measured by bali_score—an...

Some remarks on evaluating the quality of the multiple sequence alignment based on the BAliBASE benchmark

Publié en ligne: 31 déc. 2009

Pages: 675 - 678

DOI: https://doi.org/10.2478/v10006-009-0054-y

Mots clés
multiple sequence alignment, reference alignment, alignment accuracy

This content is open access.

Some remarks on evaluating the quality of the multiple sequence alignment based on the BAliBASE benchmark

Publié en ligne: 31 déc. 2009

Pages: 675 - 678

DOI: https://doi.org/10.2478/v10006-009-0054-y

Mots clésmultiple sequence alignment, reference alignment, alignment accuracy

This content is open access.

Mots clés
multiple sequence alignment, reference alignment, alignment accuracy