<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The nucleotide binding site (NBS) domain sequences were iso­lated from genomic DNA in black bamboo, using the degenerate primer designed according to the conserved motifs of the NBS resistance gene. The expected size of the PCR product was about 700 bp. Among 55 positive clones, the amino acid sequence alignment identified 33 black bamboo resistance gene analogs (RGAs) that contain the NBS conserved motifs. All of the 33 RGAs ORFs were constructed in an NJ (Neighbor-joi­ning) tree, and divided into 10 groups. This analysis demonstrated the diversity of the NBS class RGA in black bamboo. The maximum likelihood estimates of various evolutionary models were analyzed; the result showed that 2 groups with a total of 10 sequences and 12 sites demonstrated statistically significant positive selection. Most of the positive selected sites were not located in the NBS conserved motifs. Two groups of gene conversion events had been discovered, which provide a mate­rial basis and research direction in isolating black bamboo R genes.</p></abstract>

The nucleotide binding site (NBS) domain sequences were iso­lated from genomic DNA in black bamboo, using the degenerate primer designed according to the conserved motifs of the NBS resistance gene. The expected size of the PCR product was about 700 bp. Among 55 positive clones, the amino acid sequence alignment identified 33 black bamboo resistance gene analogs (RGAs) that contain the NBS conserved motifs. All of the 33 RGAs ORFs were constructed in an NJ (Neighbor-joi­ning) tree, and divided into 10 groups. This analysis demonstrated the diversity of the NBS class RGA in black bamboo. The maximum likelihood estimates of various evolutionary models were analyzed; the result showed that 2 groups with a total of 10 sequences and 12 sites demonstrated statistically significant positive selection. Most of the positive selected sites were not located in the NBS conserved motifs. Two groups of gene conversion events had been discovered, which provide a mate­rial basis and research direction in isolating black bamboo R genes.

Cloning and Molecular Evolution Analysis of NBS Class Resistance Gene Analogs in Black Bamboo (Phyllostachys nigra)

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The nucleotide binding site (NBS) domain sequences were iso­lated from genomic DNA in black bamboo, using the degenerate primer designed according to the...

Cloning and Molecular Evolution Analysis of NBS Class Resistance Gene Analogs in Black Bamboo (Phyllostachys nigra)

Publié en ligne: 13 déc. 2018

Pages: 117 - 123

DOI: https://doi.org/10.2478/sg-2018-0017

Mots clés
Black bamboo, NBS, RGA, Positive selection, Gene conversion

© 2018 Yu Li-Xia, et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Cloning and Molecular Evolution Analysis of NBS Class Resistance Gene Analogs in Black Bamboo (Phyllostachys nigra)

Publié en ligne: 13 déc. 2018

Pages: 117 - 123

DOI: https://doi.org/10.2478/sg-2018-0017

Mots clésBlack bamboo, NBS, RGA, Positive selection, Gene con­version

© 2018 Yu Li-Xia, et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
Black bamboo, NBS, RGA, Positive selection, Gene conversion