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Genetic characterisation of African swine fever virus in outbreaks in Ha Nam province, Red River Delta Region of Vietnam, and activity of antimicrobial products against virus infection in contaminated feed

Ha Thi Thanh Tran
Ha Thi Thanh Tran
, 
Anh Duc Truong
Anh Duc Truong
, 
Duc Viet Ly
Duc Viet Ly
, 
Thi Hao Vu
Thi Hao Vu
, 
Van Tuan Hoang
Van Tuan Hoang
, 
Thi Chinh Nguyen
Thi Chinh Nguyen
, 
Thi Nhu Chu
Thi Nhu Chu
, 
Thi Huyen Nguyen
Thi Huyen Nguyen
, 
Ngoc Thi Pham
Ngoc Thi Pham
, 
Tinh Nguyen
Tinh Nguyen
, 
Andrew G. Yersin
Andrew G. Yersin
 et 
Hoang Vu Dang
Hoang Vu Dang
   | 16 juin 2020
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Publié en ligne: 16 juin 2020
Pages: 207 - 213
Reçu: 11 nov. 2019
Accepté: 01 juin 2020
DOI: https://doi.org/10.2478/jvetres-2020-0041
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© 2020 H.T.T. Tran et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Detection of ASFV in a field sample of spleen tissue. A – HAD assay of the spleen homogenate; B– the real-time PCR amplification of the p72 gene
Detection of ASFV in a field sample of spleen tissue. A – HAD assay of the spleen homogenate; B– the real-time PCR amplification of the p72 gene

Fig. 2

Phylogenetic analysis of VN/Pig/HN/19 based on its partial p72 (A) and EP402R gene encoding the CD2v protein (B) genes. Numbers along branches indicate bootstrap values >70% (1,000 replicates). The black triangle indicates the ASFV isolate from this study. Scale bars indicate nucleotide substitutions per site
Phylogenetic analysis of VN/Pig/HN/19 based on its partial p72 (A) and EP402R gene encoding the CD2v protein (B) genes. Numbers along branches indicate bootstrap values >70% (1,000 replicates). The black triangle indicates the ASFV isolate from this study. Scale bars indicate nucleotide substitutions per site

Fig. 3

(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01
(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01

Fig. 4

(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01
(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01
eISSN:
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Langue:
Anglais
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Sujets de la revue:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
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