<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2019-0071_s_005">
<h3>Introduction</h3>
<p><italic>Porcine circovirus</italic> type 3 (PCV3) is a newly discovered <italic>porcine circovirus</italic>. The molecular characteristics and genetic evolution of PCV3 in Xinjiang province, China still being unclear, the aim of the study was their elucidation.</p></sec>
<sec id="j_jvetres-2019-0071_s_006">
<h3>Material and Methods</h3>
<p>A total of 393 clinical samples were collected from pigs on commercial farms in nine different regions of Xinjiang and phylogenetic analysis based on full-length <italic>Cap</italic> genes was performed.</p></sec>
<sec id="j_jvetres-2019-0071_s_007">
<h3>Results</h3>
<p>The prevalence at farm level was 100%, while in all the tested samples it was 22.39%. Nine PCV3 strains were detected in Xinjiang province and they shared 98.9–99.3% nucleotide and 97.5–100.0% <italic>Cap</italic> gene amino acid sequence identities with other epidemic strains from China and abroad. Compared with other epidemic strains of PCV3, there were 26 base mutation sites in the <italic>Cap</italic> gene in the nine Xinjiang strains, resulting in the mutation of amino acids at positions 20, 24, 75, 77, 108, 111 and 206. Phylogenetic analysis showed that these strains can be divided into two different genetic groups, to the first of which five strains affiliated and divided between subgroups 1.1 and 1.2, and to the second of which the other four strains affiliated and similarly divided between subgroups 2.1 and 2.2.</p></sec>
<sec id="j_jvetres-2019-0071_s_008">
<h3>Conclusion</h3>
<p>PCV3 circulates widely among commercial pig farms in Xinjiang province, China, and displays obvious genetic diversity. The results provide epidemiological information useful for the prevention and control of PCV3 infection in the pig industry.</p></sec></abstract>

Introduction
Porcine circovirus type 3 (PCV3) is a newly discovered porcine circovirus. The molecular characteristics and genetic evolution of PCV3 in Xinjiang province, China still being unclear, the aim of the study was their elucidation.

Material and Methods
A total of 393 clinical samples were collected from pigs on commercial farms in nine different regions of Xinjiang and phylogenetic analysis based on full-length Cap genes was performed.

Results
The prevalence at farm level was 100%, while in all the tested samples it was 22.39%. Nine PCV3 strains were detected in Xinjiang province and they shared 98.9–99.3% nucleotide and 97.5–100.0% Cap gene amino acid sequence identities with other epidemic strains from China and abroad. Compared with other epidemic strains of PCV3, there were 26 base mutation sites in the Cap gene in the nine Xinjiang strains, resulting in the mutation of amino acids at positions 20, 24, 75, 77, 108, 111 and 206. Phylogenetic analysis showed that these strains can be divided into two different genetic groups, to the first of which five strains affiliated and divided between subgroups 1.1 and 1.2, and to the second of which the other four strains affiliated and similarly divided between subgroups 2.1 and 2.2.

Conclusion
PCV3 circulates widely among commercial pig farms in Xinjiang province, China, and displays obvious genetic diversity. The results provide epidemiological information useful for the prevention and control of PCV3 infection in the pig industry.

Molecular detection and genetic diversity of <italic>porcine circovirus</italic> type 3 in commercial pig farms in Xinjiang province, China

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Molecular detection and genetic diversity of  type 3 in commercial pig farms in Xinjiang province, China

Introduction
Porcine circovirus type 3 (PCV3) is a newly discovered porcine circovirus. The molecular characteristics and genetic evolution of PCV3 in Xinjiang...

Molecular detection and genetic diversity of porcine circovirus type 3 in commercial pig farms in Xinjiang province, China

Publié en ligne: 19 déc. 2019

Pages: 481 - 488

Reçu: 07 mai 2019

Accepté: 22 nov. 2019

DOI: https://doi.org/10.2478/jvetres-2019-0071

Mots clés
type 3, molecular detection, genetic diversity, gene, China

© 2019 Q. Mengfan et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Molecular detection and genetic diversity of porcine circovirus type 3 in commercial pig farms in Xinjiang province, China

Publié en ligne: 19 déc. 2019

Pages: 481 - 488

Reçu: 07 mai 2019

Accepté: 22 nov. 2019

DOI: https://doi.org/10.2478/jvetres-2019-0071

Mots cléstype 3, molecular detection, genetic diversity, gene, China

© 2019 Q. Mengfan et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
type 3, molecular detection, genetic diversity, gene, China