<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> Avian poxvirus infections are widespread in the domestic poultry population but are also reported in wild birds. In poultry, these infections cause significant economic losses, while wild birds may be a reservoir for poxvirus which affects breeding poultry. However, wild birds may also exhibit characteristic anatomopathological changes. This study concerns the infection of wild-living great tits (<italic>Parus major</italic>) with the avian poxvirus in Poland.<bold>Material and Methods:</bold> Samples of internal organs and skin collected from great tits were homogenised and total cellular DNA was isolated. In PCR, the primers complementary to gene encoding the core protein 4b of the HP44 strain of fowl poxvirus (FPV) were used.<bold>Results:</bold> After electrophoresis in 2% agarose gel, the PCR product of 578 bp characteristic for FPV was obtained in DNA samples isolated from skin lesions and the heart. The analysis of the nucleotide sequence of the virus strain showed 99% similarity to many poxviruses previously isolated from great tits and other free birds at various sites in the world.<bold>Conclusions:</bold> This paper is the first clinically documented evidence obtained in laboratory conditions of avian poxvirus cases in great tits in Poland.</abstract>

Introduction: Avian poxvirus infections are widespread in the domestic poultry population but are also reported in wild birds. In poultry, these infections cause significant economic losses, while wild birds may be a reservoir for poxvirus which affects breeding poultry. However, wild birds may also exhibit characteristic anatomopathological changes. This study concerns the infection of wild-living great tits (Parus major) with the avian poxvirus in Poland.Material and Methods: Samples of internal organs and skin collected from great tits were homogenised and total cellular DNA was isolated. In PCR, the primers complementary to gene encoding the core protein 4b of the HP44 strain of fowl poxvirus (FPV) were used.Results: After electrophoresis in 2% agarose gel, the PCR product of 578 bp characteristic for FPV was obtained in DNA samples isolated from skin lesions and the heart. The analysis of the nucleotide sequence of the virus strain showed 99% similarity to many poxviruses previously isolated from great tits and other free birds at various sites in the world.Conclusions: This paper is the first clinically documented evidence obtained in laboratory conditions of avian poxvirus cases in great tits in Poland.

Avian poxvirus infection in Polish great tits <italic>(Parus major)</italic>

Department of Poultry Diseases National Veterinary Research Institute

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Avian poxvirus infection in Polish great tits 

Introduction: Avian poxvirus infections are widespread in the domestic poultry population but are also reported in wild birds. In poultry, these infections...

Avian poxvirus infection in Polish great tits (Parus major)

Publié en ligne: 31 déc. 2018

Pages: 427 - 430

Reçu: 10 juil. 2018

Accepté: 09 nov. 2018

DOI: https://doi.org/10.2478/jvetres-2018-0065

Mots clés
great tits, avian poxvirus, PCR, phylogenetic analysis

© 2018 Wojciech Kozdruń et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Avian poxvirus infection in Polish great tits (Parus major)

Publié en ligne: 31 déc. 2018

Pages: 427 - 430

Reçu: 10 juil. 2018

Accepté: 09 nov. 2018

DOI: https://doi.org/10.2478/jvetres-2018-0065

Mots clésgreat tits, avian poxvirus, PCR, phylogenetic analysis

© 2018 Wojciech Kozdruń et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
great tits, avian poxvirus, PCR, phylogenetic analysis