<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2018-0044_s_005_w2aab3b7c11b1b6b1aab1c16b1Aa"><h3>Introduction</h3><p>Salicylic acid is a derivative of benzoic acid and occurs in nature. The main target of this study was to develop the liquid chromatography coupled with tandem mass spectrometry technique as a method for determination of salicylic acid in feed materials and compound feed.</p></sec><sec id="j_jvetres-2018-0044_s_006_w2aab3b7c11b1b6b1aab1c16b2Aa"><h3>Material and Methods</h3><p>Salicylic acid was extracted from feed with 0.1% hydrochloric acid in methanol. Separation was achieved in 8 min in a gradient elution using 0.1% formic acid and acetonitrile. The analyte was detected using negative electrospray tandem mass spectrometry. The procedure was validated to the specifications of the European Commission Decision No. 2002/657/EC.</p></sec><sec id="j_jvetres-2018-0044_s_007_w2aab3b7c11b1b6b1aab1c16b3Aa"><h3>Results</h3><p>The validation results showed the repeatability of the method, which was evaluated at three levels (0.25, 0.5, and 1.0 mg/kg). Calibration curves for the working ranges were linear (R<sup>2</sup> 0.9911 to 0.9936), and recoveries ranged from 98.3% to 101%. The LOD and LOQ for compound feed were 0.02 and 0.05 mg/kg, respectively. Salicylic acid was found mostly in corn, and its concentrations differed depending on whether it was young or fully grown (5.30–12.8 mg/kg and 0.13–1.01 mg/kg, respectively).</p></sec><sec id="j_jvetres-2018-0044_s_008_w2aab3b7c11b1b6b1aab1c16b4Aa"><h3>Conclusions</h3><p>A sensitive and reliable method for the determination of salicylic acid in feed and compound feed using LC-MS/MS was developed.</p></sec></abstract>

IntroductionSalicylic acid is a derivative of benzoic acid and occurs in nature. The main target of this study was to develop the liquid chromatography coupled with tandem mass spectrometry technique as a method for determination of salicylic acid in feed materials and compound feed.Material and MethodsSalicylic acid was extracted from feed with 0.1% hydrochloric acid in methanol. Separation was achieved in 8 min in a gradient elution using 0.1% formic acid and acetonitrile. The analyte was detected using negative electrospray tandem mass spectrometry. The procedure was validated to the specifications of the European Commission Decision No. 2002/657/EC.ResultsThe validation results showed the repeatability of the method, which was evaluated at three levels (0.25, 0.5, and 1.0 mg/kg). Calibration curves for the working ranges were linear (R2 0.9911 to 0.9936), and recoveries ranged from 98.3% to 101%. The LOD and LOQ for compound feed were 0.02 and 0.05 mg/kg, respectively. Salicylic acid was found mostly in corn, and its concentrations differed depending on whether it was young or fully grown (5.30–12.8 mg/kg and 0.13–1.01 mg/kg, respectively).ConclusionsA sensitive and reliable method for the determination of salicylic acid in feed and compound feed using LC-MS/MS was developed.

Determination of salicylic acid in feed using LC-MS/MS

Department of Pharmacology and Toxicology

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Determination of salicylic acid in feed using LC-MS/MS"}

IntroductionSalicylic acid is a derivative of benzoic acid and occurs in nature. The main target of this study was to develop the liquid chromatography coupled...

Determination of salicylic acid in feed using LC-MS/MS

Publié en ligne: 23 oct. 2018

Pages: 303 - 307

Reçu: 28 mai 2018

Accepté: 25 sept. 2018

DOI: https://doi.org/10.2478/jvetres-2018-0044

Mots clés
salicylic acid, feed, LC-MS/MS

© 2018 E. Protasiuk and M. Olejnik, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Determination of salicylic acid in feed using LC-MS/MS

Publié en ligne: 23 oct. 2018

Pages: 303 - 307

Reçu: 28 mai 2018

Accepté: 25 sept. 2018

DOI: https://doi.org/10.2478/jvetres-2018-0044

Mots cléssalicylic acid, feed, LC-MS/MS

© 2018 E. Protasiuk and M. Olejnik, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
salicylic acid, feed, LC-MS/MS