<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Tetragonula</italic> a genus in the Meliponini tribe (Apidae), is difficult to identify due to the many cryptic species. As technology develops, molecular taxonomic studies are used to help identify species with limited morphological characteristics. This study presents an analysis of the phylogenetic relationship between several species in the <italic>Tetragonula</italic> genus based on the sequences of the cytochrome b gene. Maximum parsimony, neighbor joining, maximum likelihood and minimum evolution methods were used to construct phylogenetic trees. The sequence divergence between <italic>T. minangkabau</italic> collected from Limau Manis and Ulu Gadut is 0.8%, while between <italic>T. minangkabau</italic> with <italic>T. minangkabau</italic> forma <italic>darek</italic> 5.5%. The low sequence divergence indicated that <italic>T. minangkabau</italic> and <italic>T. minangkabau</italic> forma <italic>darek</italic> have a close phylogenetic relationship. The analysis showed that <italic>Tetragonula</italic> (<italic>T. minangkabau</italic>, <italic>T. minangkabau</italic> forma <italic>darek</italic>, <italic>T. laeviceps</italic>, <italic>T. drescheri</italic> and <italic>T. fuscobalteata</italic>) is monophyletic. A sequence divergence of 5.5% supports the separation of <italic>Tetragonula minangkabau</italic> and <italic>T. minangkabau</italic> forma <italic>darek</italic>.</p></abstract>

Tetragonula a genus in the Meliponini tribe (Apidae), is difficult to identify due to the many cryptic species. As technology develops, molecular taxonomic studies are used to help identify species with limited morphological characteristics. This study presents an analysis of the phylogenetic relationship between several species in the Tetragonula genus based on the sequences of the cytochrome b gene. Maximum parsimony, neighbor joining, maximum likelihood and minimum evolution methods were used to construct phylogenetic trees. The sequence divergence between T. minangkabau collected from Limau Manis and Ulu Gadut is 0.8%, while between T. minangkabau with T. minangkabau forma darek 5.5%. The low sequence divergence indicated that T. minangkabau and T. minangkabau forma darek have a close phylogenetic relationship. The analysis showed that Tetragonula (T. minangkabau, T. minangkabau forma darek, T. laeviceps, T. drescheri and T. fuscobalteata) is monophyletic. A sequence divergence of 5.5% supports the separation of Tetragonula minangkabau and T. minangkabau forma darek.

Phylogenetic Analysis of <italic>Tetragonula Minangkabau</italic> and Other Species Using Cytochrome B Gene

Biology Department, Faculty of Mathematic and Natural Science

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Phylogenetic Analysis of  and Other Species Using Cytochrome B Gene

Tetragonula a genus in the Meliponini tribe (Apidae), is difficult to identify due to the many cryptic species. As technology develops, molecular taxonomic...

Phylogenetic Analysis of Tetragonula Minangkabau and Other Species Using Cytochrome B Gene

Publié en ligne: 26 juin 2019

Pages: 117 - 124

Reçu: 01 août 2018

Accepté: 03 mars 2019

DOI: https://doi.org/10.2478/jas-2019-0008

Mots clés
cytochrome b, phylogenetic analysis,

© 2019 Buti Y. Christy et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Phylogenetic Analysis of Tetragonula Minangkabau and Other Species Using Cytochrome B Gene

Publié en ligne: 26 juin 2019

Pages: 117 - 124

Reçu: 01 août 2018

Accepté: 03 mars 2019

DOI: https://doi.org/10.2478/jas-2019-0008

Mots cléscytochrome b, phylogenetic analysis,

© 2019 Buti Y. Christy et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
cytochrome b, phylogenetic analysis,