<abstract xmlns="http://www.w3.org/1999/xhtml">Avermectins and spinosyns are structurally related natural products of microbial origin and belong to a new family of macrolides which are active against a vast array of invertebrate pests. In the present study, the effects of four members of macrolides; abamectin (ABM), emamectin benzoate (EMB), spinosad (SPI) and spinetoram (SPIT), on <italic>Meloidogyne incognita</italic> were investigated under <italic>in vitro</italic> and <italic>in vivo</italic> conditions. All compounds reduced egg hatching and led to high mortality of the nematode second-stage juveniles (J2). ABM showed the maximum rate of egg hatching inhibition and J2 mortality while SPIT recorded the minimum. All treatments reduced the number of galls, egg masses, eggs/egg mass in roots and J2 in the soil when compared to the control. Based on the 10 folds of the 24 h-LC50 values of J2 mortality <italic>in vitro</italic>, EMB and ABM exhibited higher percent reduction in galls (79.68 and 71.45%), egg masses (75.19 and 70.54%), eggs/egg mass (60.49 and 40.91%) and J2 in the soil (90.31 and 86.54%), respectively, compared to SPI and SPIT. Significant increase in tomato shoot height occurred in all biopesticides (10 folds) and SPIT (20 folds). SPI at 10 folds of the 24 h-LC50 values of J2 mortality <italic>in vitro,</italic> significantly increased root length while ABM at 50 folds and SPIT at 20 folds decreased root length by 5.15% and 5.88%, respectively, compared to the untreated inoculated plants. In all treatments, the dry shoot and root weights increased, compared to the untreated control. Our findings suggest that these macrolides have the ability to regulate nematode population densities and may be an alternative to classical nematicides.</abstract>

Avermectins and spinosyns are structurally related natural products of microbial origin and belong to a new family of macrolides which are active against a vast array of invertebrate pests. In the present study, the effects of four members of macrolides; abamectin (ABM), emamectin benzoate (EMB), spinosad (SPI) and spinetoram (SPIT), on Meloidogyne incognita were investigated under in vitro and in vivo conditions. All compounds reduced egg hatching and led to high mortality of the nematode second-stage juveniles (J2). ABM showed the maximum rate of egg hatching inhibition and J2 mortality while SPIT recorded the minimum. All treatments reduced the number of galls, egg masses, eggs/egg mass in roots and J2 in the soil when compared to the control. Based on the 10 folds of the 24 h-LC50 values of J2 mortality in vitro, EMB and ABM exhibited higher percent reduction in galls (79.68 and 71.45%), egg masses (75.19 and 70.54%), eggs/egg mass (60.49 and 40.91%) and J2 in the soil (90.31 and 86.54%), respectively, compared to SPI and SPIT. Significant increase in tomato shoot height occurred in all biopesticides (10 folds) and SPIT (20 folds). SPI at 10 folds of the 24 h-LC50 values of J2 mortality in vitro, significantly increased root length while ABM at 50 folds and SPIT at 20 folds decreased root length by 5.15% and 5.88%, respectively, compared to the untreated inoculated plants. In all treatments, the dry shoot and root weights increased, compared to the untreated control. Our findings suggest that these macrolides have the ability to regulate nematode population densities and may be an alternative to classical nematicides.

Investigating the <italic>in vitro</italic> and <italic>in vivo</italic> nematicidal performance of structurally related macrolides against the root-knot nematode, <italic>Meloidogyne incognita</italic>

Pesticide Chemistry and Technology Department, Faculty of Agriculture

Plant Protection Department, Faculty of Agriculture (Saba-Basha)

Central Agricultural Pesticides Laboratory

Hellenic Plant Protection Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Investigating the  and  nematicidal performance of structurally related macrolides against the root-knot nematode, 

Avermectins and spinosyns are structurally related natural products of microbial origin and belong to a new family of macrolides which are active against a...

Investigating the in vitro and in vivo nematicidal performance of structurally related macrolides against the root-knot nematode, Meloidogyne incognita

Publié en ligne: 31 janv. 2019

Pages: 24 - 37

Reçu: 10 mai 2018

Accepté: 24 déc. 2018

DOI: https://doi.org/10.2478/hppj-2019-0005

Mots clés
avermectins, biopesticides, macrolides, nematicidal activity, root-knot nematodes, spinosyns

© 2019 M.A. Radwan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Investigating the in vitro and in vivo nematicidal performance of structurally related macrolides against the root-knot nematode, Meloidogyne incognita

Publié en ligne: 31 janv. 2019

Pages: 24 - 37

Reçu: 10 mai 2018

Accepté: 24 déc. 2018

DOI: https://doi.org/10.2478/hppj-2019-0005

Mots clésavermectins, biopesticides, macrolides, nematicidal activity, root-knot nematodes, spinosyns

© 2019 M.A. Radwan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
avermectins, biopesticides, macrolides, nematicidal activity, root-knot nematodes, spinosyns