<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Newcastle disease (ND) is a highly infectious viral disease of birds caused by the Newcastle disease virus (NDV) and doves have been incriminated in previous outbreaks of the disease that have discouraged backyard poultry productions. This survey was done to detect and characterize the NDV from 184 swabs from the cloacae and pharynxes of 67 trapped laughing doves and 25 backyard poultry birds. The study utilized haemagglutination assay (HA) followed by haemagglutination inhibition (HI) tests on HA positive samples to screen field samples. Conventional reverse transcriptase polymerase chain reaction (RT-PCR) was conducted on the HI positives to characterize the NDV. This study revealed that of 134 dove samples screened, 88 (65.7 %) were HA positive. Of these HA positives subjected to HI testing, 37 (42.1 %) were HI positive. Interestingly, 21 (56.8 %) of the HI positives were also RT-PCR positive: 8 lentogenic, 12 velogenic, while one had both lentogenic and velogenic NDV. Comparatively, of the 50 chicken samples screened, 23 (46 %) were HA positive; and of these, HA positives subjected to HI testing, 16 (69.6 %) were HI positive. Only 4 (25 %) of the HI positives were RTPCR positive: 3 lentogenic and a velogenic NDV. From this study it was concluded that laughing doves were demonstrated to be infected with either lentogenic or velogenic NDV or both. The use of red blood adsorption-de-adsorption concentration of NDV enhanced the RT-PCR detection using the fusion gene primers NDV-F 4829 and NDV-R 5031. The detection of not only lentogenic but velogenic NDV in laughing doves poses a great risk to backyard poultry production.</p></abstract>

Newcastle disease (ND) is a highly infectious viral disease of birds caused by the Newcastle disease virus (NDV) and doves have been incriminated in previous outbreaks of the disease that have discouraged backyard poultry productions. This survey was done to detect and characterize the NDV from 184 swabs from the cloacae and pharynxes of 67 trapped laughing doves and 25 backyard poultry birds. The study utilized haemagglutination assay (HA) followed by haemagglutination inhibition (HI) tests on HA positive samples to screen field samples. Conventional reverse transcriptase polymerase chain reaction (RT-PCR) was conducted on the HI positives to characterize the NDV. This study revealed that of 134 dove samples screened, 88 (65.7 %) were HA positive. Of these HA positives subjected to HI testing, 37 (42.1 %) were HI positive. Interestingly, 21 (56.8 %) of the HI positives were also RT-PCR positive: 8 lentogenic, 12 velogenic, while one had both lentogenic and velogenic NDV. Comparatively, of the 50 chicken samples screened, 23 (46 %) were HA positive; and of these, HA positives subjected to HI testing, 16 (69.6 %) were HI positive. Only 4 (25 %) of the HI positives were RTPCR positive: 3 lentogenic and a velogenic NDV. From this study it was concluded that laughing doves were demonstrated to be infected with either lentogenic or velogenic NDV or both. The use of red blood adsorption-de-adsorption concentration of NDV enhanced the RT-PCR detection using the fusion gene primers NDV-F 4829 and NDV-R 5031. The detection of not only lentogenic but velogenic NDV in laughing doves poses a great risk to backyard poultry production.

Detection of Newcastle Disease Virus (NDV) in Laughing Doves and the Risk of Spread to Backyard Poultry

Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Newcastle disease (ND) is a highly infectious viral disease of birds caused by the Newcastle disease virus (NDV) and doves have been incriminated in previous...

Detection of Newcastle Disease Virus (NDV) in Laughing Doves and the Risk of Spread to Backyard Poultry

Publié en ligne: 06 oct. 2020

Pages: 1 - 12

Reçu: 16 mars 2020

Accepté: 29 mai 2020

DOI: https://doi.org/10.2478/fv-2020-0021

Mots clés
backyard poultry, laughing doves, molecular pathotyping, Newcastle disease virus

© 2020 J. U. Okpanachi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Detection of Newcastle Disease Virus (NDV) in Laughing Doves and the Risk of Spread to Backyard Poultry

Publié en ligne: 06 oct. 2020

Pages: 1 - 12

Reçu: 16 mars 2020

Accepté: 29 mai 2020

DOI: https://doi.org/10.2478/fv-2020-0021

Mots clésbackyard poultry, laughing doves, molecular pathotyping, Newcastle disease virus

© 2020 J. U. Okpanachi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
backyard poultry, laughing doves, molecular pathotyping, Newcastle disease virus