<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> The aim of this study was to characterise Acinetobacter sp. isolated from fish. Eight isolates obtained from diseased rainbow trout and common carp cultured in Poland were analysed. The isolates were identified using API 20 NE system as Acinetobacter sp. Afterwards, they were identified by sequencing 16S rDNA gene fragment. The bacteria were identified as A. johnsonii (two isolates), A. lwoffii (two isolates), A. junii/johnsonii (one isolate), A. calcoaceticus (one isolate), and Acinetobacter sp. (two isolates). The drug resistance of isolates was examined. The majority of the isolates were resistant to ampicilin, amoxicillin, and cephalothin and all demonstrated sensitivity to fluoroquinolones, except of one isolate. Two isolates were selected for the experimental infection of trout and carp to confirm their pathogenicity. Experimentally infected fish showed disease symptoms similar to those observed in fish naturally infected with these bacteria. This is the first report concerning pathogenicity of A. johnsonii for rainbow trout and A. lwoffii for common carp. These bacteria were regarded as emerging opportunistic pathogens of fish farmed in Poland. Acinetobacter strains are commonly known as microorganisms transmitting the antibiotic resistance genes. Therefore, they might have a great impact on the resistance transfer in aquaculture.</p>
</abstract>

The aim of this study was to characterise Acinetobacter sp. isolated from fish. Eight isolates obtained from diseased rainbow trout and common carp cultured in Poland were analysed. The isolates were identified using API 20 NE system as Acinetobacter sp. Afterwards, they were identified by sequencing 16S rDNA gene fragment. The bacteria were identified as A. johnsonii (two isolates), A. lwoffii (two isolates), A. junii/johnsonii (one isolate), A. calcoaceticus (one isolate), and Acinetobacter sp. (two isolates). The drug resistance of isolates was examined. The majority of the isolates were resistant to ampicilin, amoxicillin, and cephalothin and all demonstrated sensitivity to fluoroquinolones, except of one isolate. Two isolates were selected for the experimental infection of trout and carp to confirm their pathogenicity. Experimentally infected fish showed disease symptoms similar to those observed in fish naturally infected with these bacteria. This is the first report concerning pathogenicity of A. johnsonii for rainbow trout and A. lwoffii for common carp. These bacteria were regarded as emerging opportunistic pathogens of fish farmed in Poland. Acinetobacter strains are commonly known as microorganisms transmitting the antibiotic resistance genes. Therefore, they might have a great impact on the resistance transfer in aquaculture.

Acinetobacter johnsonii and Acinetobacter lwoffii - the emerging fish pathogens

Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine

University of Environmental and Life Sciences

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Acinetobacter johnsonii and Acinetobacter lwoffii - the emerging fish pathogens"}

The aim of this study was to characterise Acinetobacter sp. isolated from fish. Eight isolates obtained from diseased rainbow trout and common carp cultured in...

Acinetobacter johnsonii and Acinetobacter lwoffii - the emerging fish pathogens

Publié en ligne: 17 juin 2014

Pages: 193 - 199

Reçu: 30 oct. 2013

Accepté: 02 juin 2014

DOI: https://doi.org/10.2478/bvip-2014-0029

Mots clés
rainbow trout, common carp, Acinetobacter, emerging pathogens.

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Acinetobacter johnsonii and Acinetobacter lwoffii - the emerging fish pathogens

Publié en ligne: 17 juin 2014

Pages: 193 - 199

Reçu: 30 oct. 2013

Accepté: 02 juin 2014

DOI: https://doi.org/10.2478/bvip-2014-0029

Mots clésrainbow trout, common carp, Acinetobacter, emerging pathogens.

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
rainbow trout, common carp, Acinetobacter, emerging pathogens.