<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Doxorubicin is one of the most commonly used chemotherapeutic agents for adjuvant chemotherapy of breast cancer. In the studies focused on finding biomarkers to predict the response of the patients and tumors to the drugs used, the Twist transcription factor has been suggested as a candidate biomarker for predicting chemo-resistance of breast tumors. In this study, we aimed to investigate the relationship between TWIST transcription factor expression and the effectiveness of doxorubicin treatment on directly taken primary tumor samples from chemotherapy-naive breast cancer patients. Twenty-six primary breast tumor samples taken from 26 different breast cancer patients were included in this study. Adenosine triphosphate tumor chemo-sensitivity assay (ATP-TCA) has been used to determine tumor response to doxorubicin and real-time reverse-transcription polymerase chain reaction (RT-PCR) was used for analyzing the <italic>TWIST1</italic> gene expression of tumors. There was a significant difference in <italic>TWIST</italic> gene expression between responder and non responder tumors (<italic>p</italic> &lt;0.05). The <italic>TWIST</italic> gene expression of the drug-resistant group was higher than the responsive group. This difference was not dependent on the histopathological features of tumors. In conclusion, compatible with earlier studies that have been performed with cell lines, the current study supports the role of higher <italic>TWIST</italic> gene expression as a biomarker for predicting the response of breast tumors to chemo-therapeutic agent doxorubicin.</p></abstract>

Doxorubicin is one of the most commonly used chemotherapeutic agents for adjuvant chemotherapy of breast cancer. In the studies focused on finding biomarkers to predict the response of the patients and tumors to the drugs used, the Twist transcription factor has been suggested as a candidate biomarker for predicting chemo-resistance of breast tumors. In this study, we aimed to investigate the relationship between TWIST transcription factor expression and the effectiveness of doxorubicin treatment on directly taken primary tumor samples from chemotherapy-naive breast cancer patients. Twenty-six primary breast tumor samples taken from 26 different breast cancer patients were included in this study. Adenosine triphosphate tumor chemo-sensitivity assay (ATP-TCA) has been used to determine tumor response to doxorubicin and real-time reverse-transcription polymerase chain reaction (RT-PCR) was used for analyzing the TWIST1 gene expression of tumors. There was a significant difference in TWIST gene expression between responder and non responder tumors (p <0.05). The TWIST gene expression of the drug-resistant group was higher than the responsive group. This difference was not dependent on the histopathological features of tumors. In conclusion, compatible with earlier studies that have been performed with cell lines, the current study supports the role of higher TWIST gene expression as a biomarker for predicting the response of breast tumors to chemo-therapeutic agent doxorubicin.

<italic>TWIST1</italic> Gene expression as a biomarker for predicting primary doxorubicin resistance in breast cancer

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 Gene expression as a biomarker for predicting primary doxorubicin resistance in breast cancer

Doxorubicin is one of the most commonly used chemotherapeutic agents for adjuvant chemotherapy of breast cancer. In the studies focused on finding biomarkers...

Sample	Tumor Grade	ER	PR	HER2	Diagnosis	TWIST 1 Gene Expression Fold-Change	ATP-TCA Results
S1	3	[+]	[+]	[–]	IDC	4.25	NR
S2	2	[+]	[+]	[–]	IDC	7.85	NR
S3	3	[+]	[+]	[–]	IDC	6.70	NR
S5	3	[+]	[–]	[–]	IDC	4.26	R
S6	3	[+]	[+]	[+]	IDC	5.80	R
S7	3	[+]	[+]	[–]	IDC; multifocal	5.36	R
S9	3	[+]	[+]	[–]	IDC	4.74	R
S10	3	[+]	[+]	[+]	IDC	3.67	R
S11	3	[+]	[+]	[+]	IDC	6.06	R
S12	3	[+]	[–]	[+]	IDC	4.44	R
S13	3	[+]	[+]	[+]	mixed IDC and IDC-L	5.84	NR
S14	3	[+]	[+]	[+]	IDC; multifocal	4.51	R
S15	2	[+]	[+]	[–]	mixed IDC and IDC-L	3.87	R
S17	3	[+]	[+]	[–]	IDC	4.88	R
S18	3	[–]	[–]	[–]	IDC	5.56	R
S19	2	[+]	[+]	[–]	IDC	5.56	R
S21	2	[+]	[+]	[+]	mixed IDC and IDC-L; multifocal	5.28	NR
S22	2	[+]	[+]	[–]	IDC	4.18	R
S23	3	[–]	[–]	[–]	IDC	5.36	NR
S27	3	[+]	[+]	[–]	IDC	2.62	R
S29	NA	NA	NA	NA	IDC	4.43	NR
S34	1	[+]	[+]	[–]	invasive mucinous carcinoma of breast	7.32	NR
S54	3	[+]	[+]	[+]	IDC	3.97	NR
S55	3	[–]	[–]	[–]	IDC	2.61	R
S87	3	[–]	[–]	[–]	IDC	0.00	R
S88	3	[–]	[–]	[+]	IDC	3.82	R

TWIST1 Gene expression as a biomarker for predicting primary doxorubicin resistance in breast cancer

Article Category: Original Article

Publié en ligne: 21 déc. 2019

Pages: 25 - 30

DOI: https://doi.org/10.2478/bjmg-2019-0025

Mots clés
Biomarker, Breast cancer, Chemotherapy, Expression, gene

© 2019 Demir S, Müslümanoğlu MH, Müslümanoğlu M, Başaran S, Çalay ZZ, Aydıner A, Vogt U, Çakır T, Kadıoğlu H, Artan S, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Clinopathological characteristics, TWIST gene expression results and ATP-TCA results of samples.

TWIST1 Gene expression as a biomarker for predicting primary doxorubicin resistance in breast cancer

Article Category: Original Article

Publié en ligne: 21 déc. 2019

Pages: 25 - 30

DOI: https://doi.org/10.2478/bjmg-2019-0025

Mots clésBiomarker, Breast cancer, Chemotherapy, Expression, gene

© 2019 Demir S, Müslümanoğlu MH, Müslümanoğlu M, Başaran S, Çalay ZZ, Aydıner A, Vogt U, Çakır T, Kadıoğlu H, Artan S, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Clinopathological characteristics, TWIST gene expression results and ATP-TCA results of samples.

Mots clés
Biomarker, Breast cancer, Chemotherapy, Expression, gene