<abstract xmlns="http://www.w3.org/1999/xhtml"><p>This work was focused on the characterization of novel isolate of <italic>Trichoderma atroviride</italic> O1, found in the forest around the village of Zázrivá (the Northern Slovakia, region Orava). The isolate was identified by sequencing its <italic>internal transcribed spacer</italic> (ITS) region of rDNA. <italic>T. atroviride</italic> O1 stimulated the development of lateral roots of model plant <italic>Lepidium sativum</italic>. Simultaneously, the isolate has proved its high mycoparasitic potential as it displayed the ability to attack colonies of phytopathogenic fungi (<italic>Alternaria alternata, Fusarium culmorum, Botrytis cinerea</italic>). This isolate produced secondary metabolites, which were isolated and tested for the antimicrobial activity against gram-positive bacteria <italic>Staphylococcus epidermidis</italic> and <italic>Staphylococcus aureus.</italic> The growth of these bacteria was suppressed to 10 % and 40 %, respectively. The suppression of the growth of two <italic>Candida</italic> species was also strong (10 % growth). However, growth parameters of three phytopathogenic fungi <italic>(Alternaria alternata, Botrytis cinerea</italic> and <italic>Fusarium culmorum</italic>) were less affected (75 % growth in comparison with the control). Attempts were made to characterize secondary metabolites isolated from <italic>T. atroviride</italic> O1. Known peptaibols, 20—21 amino acid long, but also shorter peptides, were detected by MALDI-TOF mass spectrometry. Thus, this study demonstrates the plant growth promotion, strong mycoparasitic potential and antimicrobial activity of the isolate <italic>T. atroviride</italic> O1, which could be in part ascribed to the production of secondary metabolites. This isolate does have a potential in the biocontrol in eco-farming. Further study, particularly, the identification of produced secondary metabolites, is needed.</p></abstract>

This work was focused on the characterization of novel isolate of Trichoderma atroviride O1, found in the forest around the village of Zázrivá (the Northern Slovakia, region Orava). The isolate was identified by sequencing its internal transcribed spacer (ITS) region of rDNA. T. atroviride O1 stimulated the development of lateral roots of model plant Lepidium sativum. Simultaneously, the isolate has proved its high mycoparasitic potential as it displayed the ability to attack colonies of phytopathogenic fungi (Alternaria alternata, Fusarium culmorum, Botrytis cinerea). This isolate produced secondary metabolites, which were isolated and tested for the antimicrobial activity against gram-positive bacteria Staphylococcus epidermidis and Staphylococcus aureus. The growth of these bacteria was suppressed to 10 % and 40 %, respectively. The suppression of the growth of two Candida species was also strong (10 % growth). However, growth parameters of three phytopathogenic fungi (Alternaria alternata, Botrytis cinerea and Fusarium culmorum) were less affected (75 % growth in comparison with the control). Attempts were made to characterize secondary metabolites isolated from T. atroviride O1. Known peptaibols, 20—21 amino acid long, but also shorter peptides, were detected by MALDI-TOF mass spectrometry. Thus, this study demonstrates the plant growth promotion, strong mycoparasitic potential and antimicrobial activity of the isolate T. atroviride O1, which could be in part ascribed to the production of secondary metabolites. This isolate does have a potential in the biocontrol in eco-farming. Further study, particularly, the identification of produced secondary metabolites, is needed.

<italic>Trichoderma atroviride</italic>: an isolate from forest environment with secondary metabolites with high antimicrobial potential

Institute of Biochemistry and Microbiology

Slovak University of Technology in Bratislava

Acta Chimica Slovaca

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

: an isolate from forest environment with secondary metabolites with high antimicrobial potential

This work was focused on the characterization of novel isolate of Trichoderma atroviride O1, found in the forest around the village of Zázrivá (the Northern...

Trichoderma atroviride: an isolate from forest environment with secondary metabolites with high antimicrobial potential

Publié en ligne: 09 juil. 2019

Pages: 46 - 55

DOI: https://doi.org/10.2478/acs-2019-0008

Mots clés
antimicrobial activity, mycoparasitism, plant-growth promotion, secondary metabolites,

© 2019 Ján Víglaš et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Trichoderma atroviride: an isolate from forest environment with secondary metabolites with high antimicrobial potential

Publié en ligne: 09 juil. 2019

Pages: 46 - 55

DOI: https://doi.org/10.2478/acs-2019-0008

Mots clésantimicrobial activity, mycoparasitism, plant-growth promotion, secondary metabolites,

© 2019 Ján Víglaš et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
antimicrobial activity, mycoparasitism, plant-growth promotion, secondary metabolites,