<abstract xmlns="http://www.w3.org/1999/xhtml"><sec><h3>Background</h3><p>Hurthle cells of the thyroid gland are very rich in mitochondria and oxidative enzymes. As a high level oxidative metabolism may lead to higher level of oxidative stress and can be associated with an increased risk for cancer, we investigated whether common functional polymorphisms in antioxidant genes <italic>(SOD2, CAT, GPX, GSTP1</italic>, <italic>GSTM1</italic> and <italic>GSTT1</italic>) are associated with the development or clinical course of Hurthle cell thyroid carcinoma (HCTC).</p></sec><sec><h3>Methods</h3><p>A retrospective study was performed in 139 patients treated by thyroid surgery for a Hurthle cell neoplasm. HCTC, Hurthle cell thyroid adenoma (HCTA) or Hurthle cell thyroid nodule (HCTN) were diagnosed by pathomorphology. DNA was extracted from cores of histologically confirmed normal tissue obtained from formalin-fixed paraffin-embedded specimens and genotyped for investigated polymorphisms. Logistic regression was used to compare genotype distributions between patient groups.</p></sec><sec><h3>Results</h3><p>HCTC, HCTA and HCTN were diagnosed in 53, 47 and 21 patients, respectively. Metastatic disease and recurrence of HCTC were diagnosed in 20 and 16 HCTC patients, respectively. Genotypes and allele frequencies of investigated polymorphisms did not deviate from Hardy-Weinberg equilibrium in patients with HCTC, HCTA and HCTN. Under the dominant genetic model we observed no differences in the genotype frequency distribution of the investigated polymorphisms when the HCTA and HCTN group was compared to the HCTC group for diagnosis of HCTC or for the presence of metastatic disease. However, <italic>GPX1</italic> polymorphism was associated with the occurrence of recurrent disease (p = 0.040).</p></sec><sec><h3>Conclusions</h3><p><italic>GPX1</italic> polymorphism may influence the risk for recurrent disease in HCTC.</p></sec></abstract>

BackgroundHurthle cells of the thyroid gland are very rich in mitochondria and oxidative enzymes. As a high level oxidative metabolism may lead to higher level of oxidative stress and can be associated with an increased risk for cancer, we investigated whether common functional polymorphisms in antioxidant genes (SOD2, CAT, GPX, GSTP1, GSTM1 and GSTT1) are associated with the development or clinical course of Hurthle cell thyroid carcinoma (HCTC).MethodsA retrospective study was performed in 139 patients treated by thyroid surgery for a Hurthle cell neoplasm. HCTC, Hurthle cell thyroid adenoma (HCTA) or Hurthle cell thyroid nodule (HCTN) were diagnosed by pathomorphology. DNA was extracted from cores of histologically confirmed normal tissue obtained from formalin-fixed paraffin-embedded specimens and genotyped for investigated polymorphisms. Logistic regression was used to compare genotype distributions between patient groups.ResultsHCTC, HCTA and HCTN were diagnosed in 53, 47 and 21 patients, respectively. Metastatic disease and recurrence of HCTC were diagnosed in 20 and 16 HCTC patients, respectively. Genotypes and allele frequencies of investigated polymorphisms did not deviate from Hardy-Weinberg equilibrium in patients with HCTC, HCTA and HCTN. Under the dominant genetic model we observed no differences in the genotype frequency distribution of the investigated polymorphisms when the HCTA and HCTN group was compared to the HCTC group for diagnosis of HCTC or for the presence of metastatic disease. However, GPX1 polymorphism was associated with the occurrence of recurrent disease (p = 0.040).ConclusionsGPX1 polymorphism may influence the risk for recurrent disease in HCTC.

Functional polymorphisms in antioxidant genes in Hurthle cell thyroid neoplasm - an association of <italic>GPX1</italic> polymorphism and recurrent Hurthle cell thyroid carcinoma

Institute of Oncology Ljubljana, Slovenia

Pharmacogenetics Laboratory, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Slovenia

Radiology and Oncology

Functional polymorphisms in antioxidant genes in Hurthle cell thyroid neoplasm - an association of  polymorphism and recurrent Hurthle cell thyroid carcinoma

BackgroundHurthle cells of the thyroid gland are very rich in mitochondria and oxidative enzymes. As a high level oxidative metabolism may lead to higher level...

Functional polymorphisms in antioxidant genes in Hurthle cell thyroid neoplasm - an association of GPX1 polymorphism and recurrent Hurthle cell thyroid carcinoma

Article Category: Research Article

Publié en ligne: 19 juil. 2016

Pages: 289 - 296

Reçu: 04 janv. 2016

Accepté: 02 mai 2016

DOI: https://doi.org/10.1515/raon-2016-0031

© 2016 Radiol Oncol

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