<abstract xmlns="http://www.w3.org/1999/xhtml"> The parasitic nematodes of genus Trichinella are infective to a wide range of hosts, including humans, and have global distribution from tropic to arctic areas. Muscle samples from animals, collected in two areas of the Russian Federation, Chukotka Peninsula and Arkhangelsk Oblast, were tested for the presence of Trichinella spp. larvae. Trichinella spp. larvae were recovered from tissues of eleven wild and domestic animals: 1 polar bear, 1 wolverine, 3 arctic foxes, 1 ringed seal, 1 brown bear, 1 cat, 1 sled dog, 1 domestic pig and 1 northern sea lion.Two molecular methods, multiplex PCR and inter-simple sequence repeat polymerase chain reaction (ISSR PCR), were utilized for species identification. T. nativa was identified in the majority of tested animals and mixed infections with two Trichinella species were detected in four animals. T. spiralis/T. pseudospiralis mixed infection was found in a domestic pig and T. spiralis/T. nativa in two arctic foxes and a northern sea lion.Here, for the first time, Trichinella spp. infection in a northern sea lion and the northern sea lion as a new host for T. spiralis and T. nativa is reported. Discovery of Trichinella spp. infection in a new host, such as a northern sea lion, which is an opportunistic marine predator, mainly feeding on fish, indicates environmental contamination. Leaving of carcasses or waste from animals and improper management of livestock operations is important for spreading Trichinella spp. infection in free living animals. Therefore, further extensive epidemiological and environmental research and molecular studies are needed to investigate the local fauna.</abstract>

The parasitic nematodes of genus Trichinella are infective to a wide range of hosts, including humans, and have global distribution from tropic to arctic areas. Muscle samples from animals, collected in two areas of the Russian Federation, Chukotka Peninsula and Arkhangelsk Oblast, were tested for the presence of Trichinella spp. larvae. Trichinella spp. larvae were recovered from tissues of eleven wild and domestic animals: 1 polar bear, 1 wolverine, 3 arctic foxes, 1 ringed seal, 1 brown bear, 1 cat, 1 sled dog, 1 domestic pig and 1 northern sea lion.Two molecular methods, multiplex PCR and inter-simple sequence repeat polymerase chain reaction (ISSR PCR), were utilized for species identification. T. nativa was identified in the majority of tested animals and mixed infections with two Trichinella species were detected in four animals. T. spiralis/T. pseudospiralis mixed infection was found in a domestic pig and T. spiralis/T. nativa in two arctic foxes and a northern sea lion.Here, for the first time, Trichinella spp. infection in a northern sea lion and the northern sea lion as a new host for T. spiralis and T. nativa is reported. Discovery of Trichinella spp. infection in a new host, such as a northern sea lion, which is an opportunistic marine predator, mainly feeding on fish, indicates environmental contamination. Leaving of carcasses or waste from animals and improper management of livestock operations is important for spreading Trichinella spp. infection in free living animals. Therefore, further extensive epidemiological and environmental research and molecular studies are needed to investigate the local fauna.

Molecular identification of Trichinella isolates from wildlife animals of the Russian Arctic territories

Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw,

K.I. Skryabin’s All Russian Institute of Fundamental and Applied Parasitology of Animals and Plants, Agricultural Academy of Sciences, Cheremushkinskaya St. 28, 117218 Moscow,

Center of Parasitology, A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, 33 Leninskij prosp., 119071 Moscow,

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The parasitic nematodes of genus Trichinella are infective to a wide range of hosts, including humans, and have global distribution from tropic to arctic...

Molecular identification of Trichinella isolates from wildlife animals of the Russian Arctic territories

Article Category: Research Article

Publié en ligne: 09 févr. 2017

Pages: 11 - 16

Reçu: 29 sept. 2016

Accepté: 13 déc. 2016

DOI: https://doi.org/10.1515/helm-2017-0002

Mots clés
Trichinella, new host, isolates, wildlife animals, Russia

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Molecular identification of Trichinella isolates from wildlife animals of the Russian Arctic territories

Article Category: Research Article

Publié en ligne: 09 févr. 2017

Pages: 11 - 16

Reçu: 29 sept. 2016

Accepté: 13 déc. 2016

DOI: https://doi.org/10.1515/helm-2017-0002

Mots clésTrichinella, new host, isolates, wildlife animals, Russia

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
Trichinella, new host, isolates, wildlife animals, Russia