<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine.</p></abstract>

The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine.

LC-MS/MS Determination of Tropane Alkaloids in Maize Crop

Institute of Field and Vegetable Crops Novi Sad

Institute for Plant Protection and Environment

Contemporary Agriculture

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"LC-MS/MS Determination of Tropane Alkaloids in Maize Crop"}

The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic...

LC-MS/MS Determination of Tropane Alkaloids in Maize Crop

Publié en ligne: 03 janv. 2019

Pages: 221 - 226

Reçu: 05 déc. 2018

Accepté: 26 déc. 2018

DOI: https://doi.org/10.1515/contagri-2018-0032

Mots clés
tropan alkaloids, Datura spp., maize, LC-MS/MS

© 2018 Gorica Vuković et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

LC-MS/MS Determination of Tropane Alkaloids in Maize Crop

Publié en ligne: 03 janv. 2019

Pages: 221 - 226

Reçu: 05 déc. 2018

Accepté: 26 déc. 2018

DOI: https://doi.org/10.1515/contagri-2018-0032

Mots cléstropan alkaloids, Datura spp., maize, LC-MS/MS

© 2018 Gorica Vuković et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
tropan alkaloids, Datura spp., maize, LC-MS/MS