<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Bacillus spp. can be regarded as a rare component of the nasal mucosa microflora. The aim of this study was to identify Bacillus spp. from the nasal mucosa of healthy adults living in the suburban area near Lublin using the matrix-assisted laser desorptionionization time-of-flight mass spectrometry (MALDI-TOF MS) system. </p><p>A total of 11 bacterial isolates from the nasal specimens were cultured. The following species were identified using the routine microbiological methods: Staphylococcus aureus (3 isolates), S. epidermidis (1 isolate), S. intermedius (1 isolate) and Staphylococcus spp. (1 isolate). Moreover, 2 strains of Escherichia coli were isolated. Besides, 3 isolates of Bacillus spp. were found. These isolates were characterized by means of MALDI-TOF MS, resulting in highly specific mass spectral fingerprints and these were identified as B. pumilus, B. safenis and B. licheniformis. It was observed that all studied Bacillus spp. isolates only had the masses in common at 3864 ± 2, 7727 ± 2, and 14301 ± 4. The spectra of B. safensis and B. pumilus showed peaks at m/z 4914 ± 3, 6621 ± 3 and 14291 ± 2, which were absent in the spectrum of B. licheniformis. For B. safenis and B. pumilus, other potential biomarkers could be found at m/z 12620 and 16668, respectively.</p></abstract>

Bacillus spp. can be regarded as a rare component of the nasal mucosa microflora. The aim of this study was to identify Bacillus spp. from the nasal mucosa of healthy adults living in the suburban area near Lublin using the matrix-assisted laser desorptionionization time-of-flight mass spectrometry (MALDI-TOF MS) system. A total of 11 bacterial isolates from the nasal specimens were cultured. The following species were identified using the routine microbiological methods: Staphylococcus aureus (3 isolates), S. epidermidis (1 isolate), S. intermedius (1 isolate) and Staphylococcus spp. (1 isolate). Moreover, 2 strains of Escherichia coli were isolated. Besides, 3 isolates of Bacillus spp. were found. These isolates were characterized by means of MALDI-TOF MS, resulting in highly specific mass spectral fingerprints and these were identified as B. pumilus, B. safenis and B. licheniformis. It was observed that all studied Bacillus spp. isolates only had the masses in common at 3864 ± 2, 7727 ± 2, and 14301 ± 4. The spectra of B. safensis and B. pumilus showed peaks at m/z 4914 ± 3, 6621 ± 3 and 14291 ± 2, which were absent in the spectrum of B. licheniformis. For B. safenis and B. pumilus, other potential biomarkers could be found at m/z 12620 and 16668, respectively.

Identification of Bacillus spp. colonizing the nasal mucosa of healthy adults living in the suburban area using the matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) system

Department of Pharmaceutical Microbiology, Medical University of Lublin, W. Chodzki 1, 20-093 Lublin, Poland

Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences of Lublin, Gleboka 30, 20-612 Lublin, Poland

Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences of Lublin, Gleboka 30, 20-612 Lublin, Poland

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This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Bacillus spp. can be regarded as a rare component of the nasal mucosa microflora. The aim of this study was to identify Bacillus spp. from the nasal mucosa of...

Identification of Bacillus spp. colonizing the nasal mucosa of healthy adults living in the suburban area using the matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) system

Publié en ligne: 03 mars 2015

Pages: 137 - 141

Reçu: 01 oct. 2014

Accepté: 20 oct. 2014

DOI: https://doi.org/10.1515/cipms-2015-0001

Mots clés
Bacillus spp., nasal mucosa, MALDI-TOF MS

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Identification of Bacillus spp. colonizing the nasal mucosa of healthy adults living in the suburban area using the matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) system

Publié en ligne: 03 mars 2015

Pages: 137 - 141

Reçu: 01 oct. 2014

Accepté: 20 oct. 2014

DOI: https://doi.org/10.1515/cipms-2015-0001

Mots clésBacillus spp., nasal mucosa, MALDI-TOF MS

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Bacillus spp., nasal mucosa, MALDI-TOF MS