<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">Ovalbumin (OVA)-sensitization is a common way to evoke changes similar to changes in allergic asthma in humans. Activated cells produce various pro-inflammatory and vasoactive substances including reactive oxygen species. The goal of this pilot study was to evaluate mobilization of leukocytes into the lungs and oxidation processes induced by OVA-sensitization in a guinea-pig model of allergic asthma. Guinea-pigs were divided into OVA-sensitized and naïve animals. After sacrificing animals, blood samples were taken and total and differential leukocyte counts were calculated, and eosinophil cationic protein (ECP) and total antioxidant status (TAS) in the plasma were determined. Left lungs were saline-lavaged and total number of cells and differential leukocyte count in the bronchoalveolar lavage fluid (BAL) were calculated. Right lung tissue was homogenized, ECP, TAS and products of lipid and protein oxidation (thiobarbituric acid-reactive substances and lysine-lipoperoxidation end-products) were determined in the lung homogenate. OVA-sensitization increased a total number of cells and percentages of eosinophils and neutrophils and slightly increased ECP in the blood and in the BAL fluid. In addition, increased lipid and protein oxidation in the lung homogenate, and decreased TAS in the plasma was found in OVA-sensitized compared to naïve animals. In conclusion, OVA-sensitization increased mobilization of leukocytes into the lungs and elevated production of reactive oxygen spesies (ROS), accompanied by a decrease in plasma TAS.</abstract>

Department of Physiology, Jessenius Faculty of Medicine, Comenius University, Martin

Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University, Martin

Department of Clinical Biochemistry, Jessenius Faculty of Medicine, Comenius University and University Hospital, Martin

Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia

Changes in Several Inflammatory and Oxidation Markers after Ovalbumin-Sensitization in a Guinea Pig Model of Allergic Asthma - A Pilot Study

<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">The authors studied a group of healthy men of an average age 24.4±2.1 yrs (x ± SEM) before the oral intake of ethanol and 1 and 2 hours after its ingestion. The volunteers, n= 10, of an average weight 79.1 kg ± 2.7 kg (± SEM) consumed within one minute 0.42 g ethanol/kg of individual body weight diluted in water (ratio 1:3). Concentrations of alcohol in venous blood were determined using Widmark method. One hour after the peroral intake the ethanol concentration reached 0.39±0.02 g/kg, 2 hours after peroral intake it was 0.33±0.03 g/kg.Parameters of acid-base balance (ABB) from venous blood samples were monitored in probands. Compared with baseline values, the pH of blood did not change significantly. The values of pCO2 in blood were significantly decreased, even the values of actual HCO3 and standard HCO3. The decrease of base excess continued 2 hours after intake of ethanol. There were significant variations in ABB (paired t - test), however in tolerable range. The values of glycaemia, AST, ALT, GGT, and AMS before ethanol intake and 1 and 2 hours after intake of ethanol were in the reference range and they did not change significantly.The authors find important to document even "small" intake of alcohol which may be the cause of ABB shift to a pathological range. The doctors should always keep this in mind when doing the diagnostic statement. The changes of ABB due to alcohol consumption must be expected, especially if they are associated with other pathological conditions affecting the ABB.</abstract>

Department of Public Health, Jessenius Faculty of Medicine, Comenius University, Martin

Clinic of Children and Adolescents, Jessenius Faculty of Medicine, Comenius University and University Hospital, Martin, Slovakia

Health Spa Resort s.r.o., Dudince, Slovakia

Acid-Base Changes in Young Men after Oral Intake of Ethanol

<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">Despite of the fact, that comorbidity of depression and anxiety is a frequent condition in clinical practice, current psychiatric classification systems (according to WHO-ICD 10 and according to APA-DSM IV-TR) are not taking this reality into account sufficiently. The concept of anxious depression is very important for clinical practice. Recommended guidelines and algorithms of treatment based on evidence based medicine (EBM), established mainly on randomized controlled trials are designed separately for depression and separately for anxious disorders. This presents very often a significant complication in clinical practice. The aim of this article was to bring the concept of anxious depression to closer attention with highlighting of possible therapeutic approaches.</abstract>

Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin

Clinic of Psychiatry, Jessenius Faculty of Medicine, University Hospital, Comenius University, Martin

Clinic of Anesthesiology and Intensive Medicine, Jessenius Faculty of Medicine, Comenius University, University Hospital, Martin, Slovakia

Coexisting Depression and Anxiety: Classification and Treatment

<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">The authors present a case of 41-year-old patient with unicentric type of Castleman's disease in the neck imitating carotid glomus tumor. This rare condition can affect any lymph-node in the body and neck localization occurs only in 14% of cases. The case when Castleman's disease simulating chemodectoma have not been described yet in the literature. The authors give details on the course of the disease, its diagnosis and treatment and the discussion focuses on the reasons why the auxiliary imaging methods have led to the clinical diagnosis of carotid glomus tumor, and why the treatment was chosen.</abstract>

Clinic of Otorhinolaryngology and Head and Neck Surgery, Comenius University, Martin, Slovakia

Clinic of Transplant, Visceral and Vascular Surgery, Comenius University, Martin, Slovakia

Department of Pathological Anatomy, Comenius University, Martin, Slovakia

Clinic of Radiodiagnostics, Jessenius Faculty of Medicine and University Hospital Martin, Comenius University, Martin, Slovakia

Castleman's Disease Mimicking Carotid Body Tumor

<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">Type 1 diabetes comprises autoimmune-mediated and idiopathic beta-cell destruction of the pancreatic islets of Langerhans resulting to absolute insulin deficiency. Susceptibility to T1D is influenced by both genetic and environmental factors. It is generally believed that environmental agents, such as viral infections, dietary factors in early infancy or climatic influences, trigger disease development in genetically susceptible individuals. Many candidate regions for diabetes genes have been reported, the insulin, glutamic acid 65, insuloma associated antigen 2, and zinc transporter ZnT8 genes being among the most important. The destruction of b cells is mediated mainly by cellular mechanisms; antibodies seem to reflect the ongoing autoimmune process only, not being directly involved in tissue damage. They, however, appear prior to the onset of insulin deficiency what makes us to profit from in prevention of the disease.</abstract>

Department of Internal Medicine, Jessenius Faculty of Medicine and University Hospital, Comenius University, Martin

Department of Immunology, Faculty of Medicine, Comenius University, Bratislava, Slovakia

Genetic Determination and Immunopathogenesis of Type 1 Diabetes Mellitus in Humans

AHEAD OF PRINT

Volume 24 (2024): Issue 1 (April 2024)

Volume 23 (2023): Issue 3 (December 2023)

Volume 23 (2023): Issue 2 (August 2023)

Volume 23 (2023): Issue 1 (April 2023)

Volume 22 (2022): Issue 3 (December 2022)

Volume 22 (2022): Issue 2 (August 2022)

Volume 22 (2022): Issue 1 (April 2022)

Volume 21 (2021): Issue 3 (December 2021)

Volume 21 (2021): Issue 2 (August 2021)

Volume 21 (2021): Issue 1 (March 2021)

Volume 20 (2020): Issue 3 (December 2020)

Volume 20 (2020): Issue 2 (August 2020)

Volume 20 (2020): Issue 1 (April 2020)

Volume 19 (2019): Issue 3 (December 2019)

Volume 19 (2019): Issue 2 (August 2019)

Volume 19 (2019): Issue 1 (April 2019)

Volume 18 (2018): Issue 3 (December 2018)

Volume 18 (2018): Issue 2 (August 2018)

Volume 18 (2018): Issue 1 (June 2018)

Volume 17 (2017): Issue 3 (December 2017)

Volume 17 (2017): Issue 2 (August 2017)

Volume 17 (2017): Issue 1 (April 2017)

Volume 16 (2016): Issue 3 (December 2016)

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Volume 16 (2016): Issue 1 (April 2016)

Volume 15 (2015): Issue 3 (December 2015)

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Volume 15 (2015): Issue 1 (May 2015)

Volume 14 (2014): Issue 2 (September 2014)

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Volume 11 (2011): Issue 1 (March 2011)

Acta Medica Martiniana

 Acta Medica Martiniana is a medical scientific journal, first published in print form in December 2001. It is a continuation of the journal / almanac Folia Medica Martiniana (1971 - 1996). The journal‘s owner is the Jessenius Faculty of Medicine, Comenius University, Slovakia. The aims and scope of the AMM:   Dissemination of research results and scientific knowledge from all areas of medicine and nursing.  Stimulation, facilitation and supporting of publication activity for the young medical research and clinical generation. The contributions of young novice authors (PhD students and post-doctorials) are particularly welcome.   Acta Medica Martiniana (AMM) publishes, and here only in English, high quality original papers (in extenso and short-rapid communications), case reports as well as review articles.  Acta Medica Martiniana is an open-access journal, with a periodicity of publishing three times per year (Apr/Aug/Dec). It covers a wide range of basic medical disciplines, such as anatomy, histology, biochemistry, human physiology, pharmacology, etc., as well as all clinical areas incl. preventive medicine, public health and nursing. Interdisciplinary and multidisciplinary manuscripts, including papers from all areas of biomedical research, are welcome.  All articles are submitted to a peer-review process. They are peer-reviewed anonymously (double blind) by external experts pursuant to the standard evaluation criteria. Based on the reviewers opinions, the Editorial Board will decide whether a paper is to be included in the journal.  Rejection Rate:   15-30%   Archiving  Sciendo archives the contents of this journal in Portico - digital long-term preservation service of scholarly books, journals and collections.  Plagiarism Policy  The editorial board is participating in a growing community of Similarity Check System's users in order to ensure that the content published is original and trustworthy. Similarity Check is a medium that allows for comprehensive manuscripts screening, aimed to eliminate plagiarism and provide a high standard and quality peer-review process. 