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Therapeutic effects of revascularisation on the healing of free bone grafts in dogs

Jia-San Zheng
Jia-San Zheng
, 
Hong-Ri Ruan
Hong-Ri Ruan
, 
Shuang-Qiu
Shuang-Qiu
, 
Jing-Nie
Jing-Nie
, 
Kai-Wen Hou
Kai-Wen Hou
 y 
Rui-Wu
Rui-Wu
   | 24 mar 2020
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Publicado en línea: 24 mar 2020
Páginas: 175 - 180
Recibido: 15 jul 2019
Aceptado: 02 mar 2020
DOI: https://doi.org/10.2478/jvetres-2020-0023
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© 2020 J.S. Zheng et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Images of canine radius 4, 8, and 12 weeks after repair of bone defect. A, B, and C are the X-ray results for group T at 4, 8, and 12 weeks after reimplantation; D, E, and F are the X-ray results for group C at the same intervals after reimplantation
Images of canine radius 4, 8, and 12 weeks after repair of bone defect. A, B, and C are the X-ray results for group T at 4, 8, and 12 weeks after reimplantation; D, E, and F are the X-ray results for group C at the same intervals after reimplantation

Fig. 2

Histopathological observation of free bone in groups T and C at 6 and 12 weeks after reimplantation. A and C are the histological observation at weeks 6 and 12 after reimplantation surgery for group T; B and D are the equivalent histological observation for group C. A – visible bone resorption, trabecular bone, and a small number of osteoblasts (black arrow) (HE, 200×); B – visible small trabecular bone, fibrous tissue oedema at the junction (black arrow) (HE, 200×); C – a large number of osteoblasts are present (black arrow) The bone plate is arranged regularly (red arrow) (HE, 200×); D – a large amount of new bone formation (black arrow) is visible, and the bone plate is arranged regularly (red arrow) (HE, 200×)
Histopathological observation of free bone in groups T and C at 6 and 12 weeks after reimplantation. A and C are the histological observation at weeks 6 and 12 after reimplantation surgery for group T; B and D are the equivalent histological observation for group C. A – visible bone resorption, trabecular bone, and a small number of osteoblasts (black arrow) (HE, 200×); B – visible small trabecular bone, fibrous tissue oedema at the junction (black arrow) (HE, 200×); C – a large number of osteoblasts are present (black arrow) The bone plate is arranged regularly (red arrow) (HE, 200×); D – a large amount of new bone formation (black arrow) is visible, and the bone plate is arranged regularly (red arrow) (HE, 200×)

Comparison of the average scores of Lane–Sandhu X-rays at 4, 8 and 12 weeks after surgery (n = 4)

Group 4 weeks 8 weeks 12 weeks
T 4.4 ± 0.81

– P < 0.05

 7.9 ± 0.57

– P < 0.05

 11.95 ± 0.5

– P < 0.05
C 2.95 ± 0.5 4.45 ± 0.95 8.55 ± 0.95

Comparison of mean scores of Lane–Sandhu histology bone formation scores at 6 and 12 weeks after surgery (n = 2)

Group 6 weeks 12 weeks
T 8.63 ± 0.64

– P < 0.05

 11.75 ± 0.71

– P < 0.05
C 5.4 ± 0.93 8.25 ± 0.71

Comparison of five markers in two groups of different time periods (n = 4)

Time Group BSAP (ng/L) OC (ng/L) PICP (ng/L) PINP (ng/L) CTX (ng/L)
1 day before surgery T 13.71 ± 2.86 9.26 ± 4.35 40.36 ± 6.93 44.53 ± 7.88 44.83 ± 6.03
C 14.57 ± 4.23 9.83 ± 4.28 42.77 ± 5.88 42.56 ± 5.63 44.52 ± 7.87
1 week after surgery T 12.94 ± 2.01 8.93 ± 2.74 50.78 ± 7.53 47.39 ± 7.93 48.23 ± 5.19
C 13.82 ± 3.52 9.48 ± 3.38 46.63 ± 4.65 43.79 ± 4.55 45.39 ± 7.63
2 weeks after surgery T 14.29 ± 3.51 9.62 ± 2.85 58.79 ± 7.94

– P < 0.05

 55.37 ± 6.17

– P < 0.05

 54.42 ± 6.32

– P < 0.05
C 14.51 ± 4.89 9.66 ± 4.29 49.32 ± 4.58 44.93 ± 5.69 46.47 ± 4.34
3 weeks after surgery T 15.94 ± 2.42 11.35 ± 4.47 52.54 ± 5.66 48.33 ± 7.88 52.14 ± 7.63
C 13.64 ± 5.96 8.84 ± 3.23 55.52 ± 7.18 49.73 ± 6.64 49.65 ± 5.32
4 weeks after surgery T 17.36 ± 4.57

– P < 0.05

 15.37 ± 3.63

– P < 0.05

 48.73 ± 6.37 45.62 ± 7.39 50.66 ± 7.54
C 13.97 ± 4.25 9.93 ± 3.78 52.95 ± 7.88 47.65 ± 4.38 51.22 ± 7.24
5 weeks after surgery T 16.44 ± 3.51 14.87 ± 4.16 44.84 ± 5.33 46.83 ± 7.91 48.35 ± 6.93
C 15.27 ± 3.11 11.57 ± 4.31 48.44 ± 4.69 49.53 ± 5.33 47.12 ± 7.33
6 weeks after surgery T 16.42 ± 4.12 14.47 ± 2.18 46.96 ± 5.85 48.49 ± 7.16 47.72 ± 4.66
C 16.94 ± 5.88 13.22 ± 5.12 47.78 ± 6.15 44.57 ± 7.81 48.52 ± 4.39
8 weeks after surgery T 15.51 ± 2.65 12.77 ± 4.54 50.74 ± 4.53 53.26 ± 4.47

– P < 0.05

 49.28 ± 5.62
C 14.39 ± 3.83 12.26 ± 5.42 48.63 ± 5.77 45.44 ± 6.55 48.57 ± 7.94
10 weeks after surgery T 14.62 ± 3.71 10.34 ± 3.82 51.33 ± 6.12 50.27 ± 6.97 48.28 ± 6.63
C 15.24 ± 2.79 11.73 ± 5.15 52.73 ± 5.97 52.65 ± 6.27 49.21 ± 6.88
12 weeks after surgery T 15.82 ± 4.97 9.66 ± 4.45 48.58 ± 4.78 47.88 ± 5.96 52.75 ± 6.19
C 14.78 ± 4.37 11.13 ± 3.77 51.14 ± 4.31 51.18 ± 7.91 51.36 ± 6.35
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