<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Fungal diseases are a persistent problem in the cultivation of white button mushrooms (<italic>Agaricus bisporus</italic>). The chemical control of pathogens is becoming less effective and less desirable, so new ways to limit these infections are urgently required. What is more, the disease is mostly controlled through cultural practices and good hygiene on mushroom farms. The aim of this study was to evaluate the fungicidal effects of ozone on fungal pathogens of common mushrooms. Experiments with the use of ozone gas for disinfection of growing rooms after the completion of the mushroom growing cycle were carried out. The fungicidal effectiveness of ozone fumigation was evaluated on the basis of the survival rate of the spores of the pathogens tested (<italic>Lecanicillium fungicola</italic>, <italic>Cladobotryum dendroides</italic>, <italic>Mycogone perniciosa</italic>, and <italic>Trichoderma aggressivum</italic>). Spore suspension was applied to aluminum plates and then was exposed to gaseous ozone. The assessment of the growth of colonies of fungal isolates obtained from infected surfaces was carried out using Rodac contact test plates. The results showed that <italic>L. fungicola</italic>, <italic>M. perniciosa</italic>, and <italic>C. dendroides</italic> isolates were sensitive to the gas ozone. In order to achieve 100% efficacy against <italic>Mycogone</italic> strains, a minimum of 6 hours of ozonation had to be applied, whereas for <italic>Cladobotryum</italic> strains, a minimum of 8 hours had to be applied. The <italic>Lecanicillium</italic> species was the most sensitive to ozonation because 30 minutes of ozonation was enough to gain 100% inhibition of its growth. No satisfactory results were obtained in the case of the pathogenic species of <italic>Trichoderma</italic>, regardless of the experimental conditions. Nevertheless, this study has demonstrated the usefulness of ozone as a disinfectant for empty growing rooms after the completion of the mushrooms’ cultivation cycle.</p></abstract>

Fungal diseases are a persistent problem in the cultivation of white button mushrooms (Agaricus bisporus). The chemical control of pathogens is becoming less effective and less desirable, so new ways to limit these infections are urgently required. What is more, the disease is mostly controlled through cultural practices and good hygiene on mushroom farms. The aim of this study was to evaluate the fungicidal effects of ozone on fungal pathogens of common mushrooms. Experiments with the use of ozone gas for disinfection of growing rooms after the completion of the mushroom growing cycle were carried out. The fungicidal effectiveness of ozone fumigation was evaluated on the basis of the survival rate of the spores of the pathogens tested (Lecanicillium fungicola, Cladobotryum dendroides, Mycogone perniciosa, and Trichoderma aggressivum). Spore suspension was applied to aluminum plates and then was exposed to gaseous ozone. The assessment of the growth of colonies of fungal isolates obtained from infected surfaces was carried out using Rodac contact test plates. The results showed that L. fungicola, M. perniciosa, and C. dendroides isolates were sensitive to the gas ozone. In order to achieve 100% efficacy against Mycogone strains, a minimum of 6 hours of ozonation had to be applied, whereas for Cladobotryum strains, a minimum of 8 hours had to be applied. The Lecanicillium species was the most sensitive to ozonation because 30 minutes of ozonation was enough to gain 100% inhibition of its growth. No satisfactory results were obtained in the case of the pathogenic species of Trichoderma, regardless of the experimental conditions. Nevertheless, this study has demonstrated the usefulness of ozone as a disinfectant for empty growing rooms after the completion of the mushrooms’ cultivation cycle.

Efficiency of Gaseous Ozone in Disinfection of Mushroom Growing Rooms

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Efficiency of Gaseous Ozone in Disinfection of Mushroom Growing Rooms"}

Fungal diseases are a persistent problem in the cultivation of white button mushrooms (Agaricus bisporus). The chemical control of pathogens is becoming less...

Efficiency of Gaseous Ozone in Disinfection of Mushroom Growing Rooms

Publicado en línea: 31 dic 2020

Páginas: 91 - 100

Recibido: 01 mar 2020

Aceptado: 01 sept 2020

DOI: https://doi.org/10.2478/johr-2020-0017

Palabras clave
fungal pathogens, ozonation, control

© 2020 Joanna Szumigaj-Tarnowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Efficiency of Gaseous Ozone in Disinfection of Mushroom Growing Rooms

Publicado en línea: 31 dic 2020

Páginas: 91 - 100

Recibido: 01 mar 2020

Aceptado: 01 sept 2020

DOI: https://doi.org/10.2478/johr-2020-0017

Palabras clavefungal pathogens, ozonation, control

© 2020 Joanna Szumigaj-Tarnowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
fungal pathogens, ozonation, control