<abstract xmlns="http://www.w3.org/1999/xhtml"><italic>Merremia quinquefolia</italic>, is an important medicinal plant of the family Convolvulaceae known for its vasoconstrictor, uterotonic, neurohormonic, sympathicolytic and sedative effects. In the present investigation effect of cytokinins 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ), at concentrations 1.0, 2.0, 3.0, 4.0 and 5.0 mg·dm−3 on <italic>in vitro</italic> shoot multiplication from nodal explants of <italic>M. quinquefolia</italic> was evaluated. Bud breaking and emergence of shoots started within 10-15 days of inoculation in all media containing cytokinin. Murashige and Skoog (MS) medium supplemented with 4.0 mg·dm−3 BAP resulted in maximum number of shoots from single node within 45 days. <italic>In vitro</italic> raised shoots were successfully rooted on ½ mineral salts of MS medium with 3% sucrose supplemented with 2.0 mg·dm−3 indole-3-butyric acid (IBA). This is the first report on <italic>in vitro</italic> propagation of <italic>Merremia quinquefolia</italic>. This study can be useful for development of micropropagation protocols for related taxa.</abstract>

Merremia quinquefolia, is an important medicinal plant of the family Convolvulaceae known for its vasoconstrictor, uterotonic, neurohormonic, sympathicolytic and sedative effects. In the present investigation effect of cytokinins 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ), at concentrations 1.0, 2.0, 3.0, 4.0 and 5.0 mg·dm−3 on in vitro shoot multiplication from nodal explants of M. quinquefolia was evaluated. Bud breaking and emergence of shoots started within 10-15 days of inoculation in all media containing cytokinin. Murashige and Skoog (MS) medium supplemented with 4.0 mg·dm−3 BAP resulted in maximum number of shoots from single node within 45 days. In vitro raised shoots were successfully rooted on ½ mineral salts of MS medium with 3% sucrose supplemented with 2.0 mg·dm−3 indole-3-butyric acid (IBA). This is the first report on in vitro propagation of Merremia quinquefolia. This study can be useful for development of micropropagation protocols for related taxa.

Micropropagation Of <italic>Merremia Quinquefolia</italic> (L.) Hallier F. From Nodal Explants

B.R. Doshi School of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Vallabh Vidyanagar-388120, Gujarat, India

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Micropropagation Of  (L.) Hallier F. From Nodal Explants

Merremia quinquefolia, is an important medicinal plant of the family Convolvulaceae known for its vasoconstrictor, uterotonic, neurohormonic, sympathicolytic...

Micropropagation Of Merremia Quinquefolia (L.) Hallier F. From Nodal Explants

Publicado en línea: 23 sept 2015

Páginas: 13 - 16

Recibido: 01 feb 2015

Aceptado: 01 may 2015

DOI: https://doi.org/10.2478/johr-2015-0002

Palabras clave
axillary shoot multiplication, Convolvulaceae,

© Mafatlal M. Kher et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Micropropagation Of Merremia Quinquefolia (L.) Hallier F. From Nodal Explants

Publicado en línea: 23 sept 2015

Páginas: 13 - 16

Recibido: 01 feb 2015

Aceptado: 01 may 2015

DOI: https://doi.org/10.2478/johr-2015-0002

Palabras claveaxillary shoot multiplication, Convolvulaceae,

© Mafatlal M. Kher et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
axillary shoot multiplication, Convolvulaceae,