<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Sexually transmitted diseases (STDs) are a very important cause of illness worldwide and prolonged, untreated infections with STD pathogens may have serious consequences. Our study aims to evaluate the distribution of six different STDs (Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium) in male urine samples. First void urine samples from 52 symptomatic patients were collected between April 2014 and April 2015. DNA was extracted, purified and amplified via multiplex polymerase chain reaction (PCR) for the detection of the six STD pathogens, further identified using a 2% agarose gel electrophoresis with ethidium bromide as staining agent. STD frequency in the study group was 53.84 % (28 patients), mostly in the 20-29 years age group. Among positive patients, six presented multiple infections. 35 positive DNA samples were identified in the study: 17 of C. trachomatis, 9 of U. urealyticum, 7 of N. gonorrhoeae and 2 of M. genitalium. Wide scale application of the system based on the simultaneous detection of these six pathogens inducing STD may facilitate diagnosis, especially in multiple infections.</p></abstract>

Sexually transmitted diseases (STDs) are a very important cause of illness worldwide and prolonged, untreated infections with STD pathogens may have serious consequences. Our study aims to evaluate the distribution of six different STDs (Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium) in male urine samples. First void urine samples from 52 symptomatic patients were collected between April 2014 and April 2015. DNA was extracted, purified and amplified via multiplex polymerase chain reaction (PCR) for the detection of the six STD pathogens, further identified using a 2% agarose gel electrophoresis with ethidium bromide as staining agent. STD frequency in the study group was 53.84 % (28 patients), mostly in the 20-29 years age group. Among positive patients, six presented multiple infections. 35 positive DNA samples were identified in the study: 17 of C. trachomatis, 9 of U. urealyticum, 7 of N. gonorrhoeae and 2 of M. genitalium. Wide scale application of the system based on the simultaneous detection of these six pathogens inducing STD may facilitate diagnosis, especially in multiple infections.

Distribution of sexually transmitted diseases in a group of symptomatic male patients using urine samples and PCR technique / Distribuția bolilor cu transmitere sexuală într-un grup de barbați simptomatici utilizand probe de urina si tehnica PCR

“Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

“Iuliu Haţieganu” University of Medicine and Pharmacy, Department of Microbiology, Cluj-Napoca, Romania

“Iuliu Haţieganu” University of Medicine and Pharmacy, Department of Dermatology, Cluj-Napoca, Romania

“Iuliu Haţieganu” University of Medicine and Pharmacy, Department of Cell and Molecular Biology, Cluj-Napoca, Romania

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Sexually transmitted diseases (STDs) are a very important cause of illness worldwide and prolonged, untreated infections with STD pathogens may have serious...

Distribution of sexually transmitted diseases in a group of symptomatic male patients using urine samples and PCR technique / Distribuția bolilor cu transmitere sexuală într-un grup de barbați simptomatici utilizand probe de urina si tehnica PCR

Publicado en línea: 30 sept 2015

Páginas: 323 - 331

Recibido: 29 abr 2015

Aceptado: 18 jul 2015

DOI: https://doi.org/10.1515/rrlm-2015-0029

© by Mihaela Laura Vica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.