<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The rising attention globally on the use of oats and the beneficial effect of oat compounds in nutrition has also increased interest in oat production in Latvia. The aim of this study was to evaluate protein, amino acid and gluten content in husked and hulless oat grains grown in organic and conventional farming systems. Two hulless oat (Avena sativa L.) genotypes - the breeding line '33793' and the variety 'Stendes Emilija' and one husked oat variety 'Lizete' from the State Stende Cereal Breeding Institute - were cultivated in 2013 under conventional farming methods using three nitrogen (N) application rates (80, 120, and 160 kg·ha<sup>-1</sup>) and under organic farming. Protein content was determined by Kjeldahl method, amino acid composition by high-performance liquid chromatography method using Waters AccQ Tag, and gluten content by Sandwich R5 ELISA. The results showed that oat genotype had significant effect p &lt; 0.001) on protein and gluten content, as well as on amino acid composition. The applied amount of fertiliser did not have significant effect on the studied quality parameters, but the growing system did (p &lt; 0.001). Higher content of protein was observed in hulless oat samples, compared to that in husked oat samples. There was also a significant difference (p = 0.01) in the total amount of amino acids between husked and hulless oat samples. In hulless oat variety 'Stendes Emilija' and hulless breeding line '33793' the content of gluten was similar and two times higher than in the husked oat variety 'Lizete'. Further breeding work is necessary to obtain oats with a lower content of gluten-like proteins.</p></abstract>

The rising attention globally on the use of oats and the beneficial effect of oat compounds in nutrition has also increased interest in oat production in Latvia. The aim of this study was to evaluate protein, amino acid and gluten content in husked and hulless oat grains grown in organic and conventional farming systems. Two hulless oat (Avena sativa L.) genotypes - the breeding line '33793' and the variety 'Stendes Emilija' and one husked oat variety 'Lizete' from the State Stende Cereal Breeding Institute - were cultivated in 2013 under conventional farming methods using three nitrogen (N) application rates (80, 120, and 160 kg·ha-1) and under organic farming. Protein content was determined by Kjeldahl method, amino acid composition by high-performance liquid chromatography method using Waters AccQ Tag, and gluten content by Sandwich R5 ELISA. The results showed that oat genotype had significant effect p < 0.001) on protein and gluten content, as well as on amino acid composition. The applied amount of fertiliser did not have significant effect on the studied quality parameters, but the growing system did (p < 0.001). Higher content of protein was observed in hulless oat samples, compared to that in husked oat samples. There was also a significant difference (p = 0.01) in the total amount of amino acids between husked and hulless oat samples. In hulless oat variety 'Stendes Emilija' and hulless breeding line '33793' the content of gluten was similar and two times higher than in the husked oat variety 'Lizete'. Further breeding work is necessary to obtain oats with a lower content of gluten-like proteins.

Protein, Amino Acid and Gluten Content in Oat (Avena Sativa L.) Grown in Latvia

State Stende Cereal Breeding Institute, „Dižzemes”, Dižstende, Lībagi Civil Parish, Talsi County, LV-3258, LATVIA

Faculty of Food Technology, Latvia University of Agriculture, Jelgava, LV-3001, LATVIA

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Protein, Amino Acid and Gluten Content in Oat (Avena Sativa L.) Grown in Latvia"}

The rising attention globally on the use of oats and the beneficial effect of oat compounds in nutrition has also increased interest in oat production in...

Protein, Amino Acid and Gluten Content in Oat (Avena Sativa L.) Grown in Latvia

Publicado en línea: 06 oct 2015

Páginas: 170 - 177

Recibido: 11 jun 2015

DOI: https://doi.org/10.1515/prolas-2015-0025

Palabras clave
oat, protein, amino acid, gluten, farming systems

© by Laila Vilmane

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Protein, Amino Acid and Gluten Content in Oat (Avena Sativa L.) Grown in Latvia

Publicado en línea: 06 oct 2015

Páginas: 170 - 177

Recibido: 11 jun 2015

DOI: https://doi.org/10.1515/prolas-2015-0025

Palabras claveoat, protein, amino acid, gluten, farming systems

© by Laila Vilmane

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
oat, protein, amino acid, gluten, farming systems