<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Matrix metalloproteinases (MMPs) areagroup of proteases containing Zn ions asacofactor, which are involved in degrading ofalarge number of extracellular matrix proteins, and bioactive molecules. They also playamajor role in processes such as cell proliferation, cell migration, differentiation and apoptosis. Very little is known about the expression and function of MMPs in the male reproductive tract. Occurrence of MMP-2 and MMP-9 activity in human seminal plasma has been previously reported but their origin and function are still not fully understood. The aim of this study was to examine the presence of MMP-2 and MMP-9 in normal and abnormal human sperm samples and find if any correlation existed between the levels of expression of MMPs and fertilization potential of the spermatozoa. Human spermsamples were examined for the presence of MMP-2 and MMP-9 by gel zymography and western blot analysis. A DNAfragmentation test was performed. The samples were divided into two groups - samples with normozoospermia and teratozoospermia. The gelatin zymography showed gelatinolytic bands with molecular weight 64 and 72 k Da corresponding to active and inactive form of MMP-2. MMP-9 was not detected. The MMP-2 enzymatic activity appeared to be much higher in samples with compromised sperm morphology as compared to the normozoospermic samples. The mean DNAfragmentation index (DFI) of the group with teratozoospermia was relatively higher (22.16%) and over the upper reference limits, compared to the normozoospermic group, in which it was within the normal range (17.26%).</p></abstract>

Matrix metalloproteinases (MMPs) areagroup of proteases containing Zn ions asacofactor, which are involved in degrading ofalarge number of extracellular matrix proteins, and bioactive molecules. They also playamajor role in processes such as cell proliferation, cell migration, differentiation and apoptosis. Very little is known about the expression and function of MMPs in the male reproductive tract. Occurrence of MMP-2 and MMP-9 activity in human seminal plasma has been previously reported but their origin and function are still not fully understood. The aim of this study was to examine the presence of MMP-2 and MMP-9 in normal and abnormal human sperm samples and find if any correlation existed between the levels of expression of MMPs and fertilization potential of the spermatozoa. Human spermsamples were examined for the presence of MMP-2 and MMP-9 by gel zymography and western blot analysis. A DNAfragmentation test was performed. The samples were divided into two groups - samples with normozoospermia and teratozoospermia. The gelatin zymography showed gelatinolytic bands with molecular weight 64 and 72 k Da corresponding to active and inactive form of MMP-2. MMP-9 was not detected. The MMP-2 enzymatic activity appeared to be much higher in samples with compromised sperm morphology as compared to the normozoospermic samples. The mean DNAfragmentation index (DFI) of the group with teratozoospermia was relatively higher (22.16%) and over the upper reference limits, compared to the normozoospermic group, in which it was within the normal range (17.26%).

Original Article. Matrix Metalloproteinase Mmp-2 and Mmp-9 Activities in Seminal Plasma

MC Clinical Institute for Reproductive Medicine 20, Skobelev Blvd. Pleven, 5800,

MC Clinical Institute for Reproductive Medicine–Pleven,

Department of Anatomy, Histology, Cytology and Biology, Medical University–Pleven,

Institute of Biology and Immunology of Reproduction“Acad. Kiril Bratanov”, Department of Immunobiology of Reproduction, Bulgarian Academy of Sciences,

Journal of Biomedical and Clinical Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Matrix metalloproteinases (MMPs) areagroup of proteases containing Zn ions asacofactor, which are involved in degrading ofalarge number of extracellular matrix...

Original Article. Matrix Metalloproteinase Mmp-2 and Mmp-9 Activities in Seminal Plasma

Publicado en línea: 06 abr 2017

Páginas: 114 - 120

Recibido: 29 sept 2016

Aceptado: 20 dic 2016

DOI: https://doi.org/10.1515/jbcr-2016-0016

Palabras clave
sperm quality parameters, infertility, DNAdamage

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Original Article. Matrix Metalloproteinase Mmp-2 and Mmp-9 Activities in Seminal Plasma

Publicado en línea: 06 abr 2017

Páginas: 114 - 120

Recibido: 29 sept 2016

Aceptado: 20 dic 2016

DOI: https://doi.org/10.1515/jbcr-2016-0016

Palabras clavesperm quality parameters, infertility, DNAdamage

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Palabras clave
sperm quality parameters, infertility, DNAdamage