<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The purpose of this study was to assess the activity of <italic>Bacillus subtilis</italic> BS-2 and peppermint oil against <italic>Botrytis cinerea</italic>. In this study parameters such as the age and the density of the bacterial culture and the incubation temperature were taken into consideration. Furthermore, the cellulolytic activity of the bacterium was determined. The effect of peppermint oil was evaluated at a concentration range of 0.5-4.0 %. The research was conducted with a dual culture plate method. The influence of <italic>B. subtilis</italic> BS-2 and peppermint oil on the growth of <italic>B. cinerea</italic> was evaluated based on the growth rate index. It was noted that the bacterial culture occurred at an initial density of <italic>OD</italic><sub>560</sub> = 1.0, cultivated at 30 °C for 48 hours demonstrated the strongest antagonistic effect (57.07 % inhibition). Furthermore, it was observed that the highest cellulolytic activity occurred on the bacteria incubated for 48 hours at 37 °C. The effect of mint oil, at the lowest concentration of 0.5-1.0 %, was much weaker on bacterial activity (1.1-12.1 % inhibition). The highest concentration (4.0 %) of mint oil caused the maximum inhibition (31.9 %) of the mycelial growth. <italic>B. subtilis</italic> BS-2 may be environmental-friendly alternatives for protecting plants against <italic>B. cinerea</italic></p></abstract>

The purpose of this study was to assess the activity of Bacillus subtilis BS-2 and peppermint oil against Botrytis cinerea. In this study parameters such as the age and the density of the bacterial culture and the incubation temperature were taken into consideration. Furthermore, the cellulolytic activity of the bacterium was determined. The effect of peppermint oil was evaluated at a concentration range of 0.5-4.0 %. The research was conducted with a dual culture plate method. The influence of B. subtilis BS-2 and peppermint oil on the growth of B. cinerea was evaluated based on the growth rate index. It was noted that the bacterial culture occurred at an initial density of OD560 = 1.0, cultivated at 30 °C for 48 hours demonstrated the strongest antagonistic effect (57.07 % inhibition). Furthermore, it was observed that the highest cellulolytic activity occurred on the bacteria incubated for 48 hours at 37 °C. The effect of mint oil, at the lowest concentration of 0.5-1.0 %, was much weaker on bacterial activity (1.1-12.1 % inhibition). The highest concentration (4.0 %) of mint oil caused the maximum inhibition (31.9 %) of the mycelial growth. B. subtilis BS-2 may be environmental-friendly alternatives for protecting plants against B. cinerea

<italic>Bacillus subtilis</italic> BS-2 and Peppermint Oil as Biocontrol Agents Against <italic>Botrytis cinerea</italic>

Ecological Chemistry and Engineering S

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 BS-2 and Peppermint Oil as Biocontrol Agents Against 

The purpose of this study was to assess the activity of Bacillus subtilis BS-2 and peppermint oil against Botrytis cinerea. In this study parameters such as...

Bacillus subtilis BS-2 and Peppermint Oil as Biocontrol Agents Against Botrytis cinerea

Publicado en línea: 11 oct 2019

Páginas: 597 - 607

DOI: https://doi.org/10.1515/eces-2019-0044

Palabras clave
BS-2, CMCase activity, peppermint oil, antifungal activity,

© 2019 Katarzyna Grata et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Bacillus subtilis BS-2 and Peppermint Oil as Biocontrol Agents Against Botrytis cinerea

Publicado en línea: 11 oct 2019

Páginas: 597 - 607

DOI: https://doi.org/10.1515/eces-2019-0044

Palabras claveBS-2, CMCase activity, peppermint oil, antifungal activity,

© 2019 Katarzyna Grata et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
BS-2, CMCase activity, peppermint oil, antifungal activity,