<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Mesenchymal stem cell (MSC) differentiation is regulated intrinsically by transcription factors and extrinsically by the extracellular matrix. We tested whether matrix metalloproteinase-2 (MMP-2) and its inhibitor TIMP-2, MEF2a and TAZ transcription factors are involved in porcine MSC differentiation towards adipocytes and osteocytes. Flow cytometry and immunofluorescence were used to investigate the expression levels of multipotent cell surface markers CD73 and CD105. Real- time PCR was performed to detect the osteogenic- and adipogenic-specific markers, osteocalcin and aP2, respectively, and to estimate the MMP-2, TIMP-2, MEF2a and TAZ transcript expression levels in three groups of cell, i.e., undifferentiated MSCs, adipocytes (A) and osteocytes (O). We showed that at the transcript level, the differentiation of MSCs towards adipocyte fate may involve MMP-2, TIMP-2 and TAZ. We also show that the differentiation of MSCs toward osteocyte fate may involve TIMP-2, MEF2a and TAZ. Our research provides preliminary data on the possible role of the MMP-2, TIMP-2 and TAZ transcripts in adipogenic differentiation and of the TIMP-2, TAZ and MEF2a transcripts in the osteogenic differentiation of porcine MSCs. We report for the first time the possible involvement of MEF2a in the osteogenesis of porcine MSCs. Our work may provide additional evidence for the MMP-independent function of TIMP-2 during osteogenesis.</p></abstract>

Mesenchymal stem cell (MSC) differentiation is regulated intrinsically by transcription factors and extrinsically by the extracellular matrix. We tested whether matrix metalloproteinase-2 (MMP-2) and its inhibitor TIMP-2, MEF2a and TAZ transcription factors are involved in porcine MSC differentiation towards adipocytes and osteocytes. Flow cytometry and immunofluorescence were used to investigate the expression levels of multipotent cell surface markers CD73 and CD105. Real- time PCR was performed to detect the osteogenic- and adipogenic-specific markers, osteocalcin and aP2, respectively, and to estimate the MMP-2, TIMP-2, MEF2a and TAZ transcript expression levels in three groups of cell, i.e., undifferentiated MSCs, adipocytes (A) and osteocytes (O). We showed that at the transcript level, the differentiation of MSCs towards adipocyte fate may involve MMP-2, TIMP-2 and TAZ. We also show that the differentiation of MSCs toward osteocyte fate may involve TIMP-2, MEF2a and TAZ. Our research provides preliminary data on the possible role of the MMP-2, TIMP-2 and TAZ transcripts in adipogenic differentiation and of the TIMP-2, TAZ and MEF2a transcripts in the osteogenic differentiation of porcine MSCs. We report for the first time the possible involvement of MEF2a in the osteogenesis of porcine MSCs. Our work may provide additional evidence for the MMP-independent function of TIMP-2 during osteogenesis.

5. MMP-2, TIMP-2, TAZ and MEF2a Transcript Expression in Osteogenic and Adipogenic Differentiation of Porcine Mesenchymal Stem Cells

Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland

Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, 60-632 Poznań, Poland

Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, 60-627 Poznań, Poland

Foundation of Cardiac Surgery Development, 41-800 Zabrze, Poland

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mesenchymal stem cell (MSC) differentiation is regulated intrinsically by transcription factors and extrinsically by the extracellular matrix. We tested...

5. MMP-2, TIMP-2, TAZ and MEF2a Transcript Expression in Osteogenic and Adipogenic Differentiation of Porcine Mesenchymal Stem Cells

Article Category: Animal Genetics and Breeding

Publicado en línea: 06 may 2016

Páginas: 369 - 385

Recibido: 30 ene 2015

Aceptado: 24 sept 2015

DOI: https://doi.org/10.1515/aoas-2015-0065

Palabras clave
mesenchymal stem cells, TIMP-2, TAZ, MEF2a, transcripts, osteogenic, adipogenic, differentiation

© by Jolanta Opiela

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

5. MMP-2, TIMP-2, TAZ and MEF2a Transcript Expression in Osteogenic and Adipogenic Differentiation of Porcine Mesenchymal Stem Cells

Article Category: Animal Genetics and Breeding

Publicado en línea: 06 may 2016

Páginas: 369 - 385

Recibido: 30 ene 2015

Aceptado: 24 sept 2015

DOI: https://doi.org/10.1515/aoas-2015-0065

Palabras clavemesenchymal stem cells, TIMP-2, TAZ, MEF2a, transcripts, osteogenic, adipogenic, differentiation

© by Jolanta Opiela

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Palabras clave
mesenchymal stem cells, TIMP-2, TAZ, MEF2a, transcripts, osteogenic, adipogenic, differentiation