<abstract xmlns="http://www.w3.org/1999/xhtml">Dielectrophoresis (DEP) is a label-free technique for the characterization and manipulation of biological particles - such as cells, bacteria and viruses. Many studies have focused on the DEP cross-over frequency <italic>f</italic>xo1, where cells in a non-uniform electric field undergo a transition from negative to positive DEP. Determination of <italic>f</italic>xo1 provides a value for the membrane capacitance from the cell diameter, the means to monitor changes in cell morphology and viability, and the information required when devising DEP cell separation protocols. In this paper we describe the first systematic measurements of the second DEP cross-over frequency <italic>f</italic>xo2 that occurs at much higher frequencies. Theory indicates that <italic>f</italic>xo2 is sensitive to the internal dielectric properties of a cell, and our experiments on murine myeloma cells reveal that these properties exhibit temporal changes that are sensitive to both the osmolality and temperature of the cell suspending medium.</abstract>

Dielectrophoresis (DEP) is a label-free technique for the characterization and manipulation of biological particles - such as cells, bacteria and viruses. Many studies have focused on the DEP cross-over frequency fxo1, where cells in a non-uniform electric field undergo a transition from negative to positive DEP. Determination of fxo1 provides a value for the membrane capacitance from the cell diameter, the means to monitor changes in cell morphology and viability, and the information required when devising DEP cell separation protocols. In this paper we describe the first systematic measurements of the second DEP cross-over frequency fxo2 that occurs at much higher frequencies. Theory indicates that fxo2 is sensitive to the internal dielectric properties of a cell, and our experiments on murine myeloma cells reveal that these properties exhibit temporal changes that are sensitive to both the osmolality and temperature of the cell suspending medium.

Dielectrophoretic Characterisation of Mammalian Cells above 100 MHz

Journal of Electrical Bioimpedance

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Dielectrophoretic Characterisation of Mammalian Cells above 100 MHz"}

Dielectrophoresis (DEP) is a label-free technique for the characterization and manipulation of biological particles - such as cells, bacteria and viruses. Many...

Dielectrophoretic Characterisation of Mammalian Cells above 100 MHz

Article Category: Articles

Online veröffentlicht: 07. Sept. 2011

Seitenbereich: 64 - 71

Eingereicht: 22. Juli 2011

DOI: https://doi.org/10.5617/jeb.196

Schlüsselwörter
Biodielectrics, Cytoplasm Conductivity, Dielectrophoresis, Myeloma Cells, Nucleus conductivity

© 2011 Colin Chung, Martin Waterfall, Steve Pells, Anoop Menachery, Stewart Smith, Ronald Pethig, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Dielectrophoretic Characterisation of Mammalian Cells above 100 MHz

Article Category: Articles

Online veröffentlicht: 07. Sept. 2011

Seitenbereich: 64 - 71

Eingereicht: 22. Juli 2011

DOI: https://doi.org/10.5617/jeb.196

SchlüsselwörterBiodielectrics, Cytoplasm Conductivity, Dielectrophoresis, Myeloma Cells, Nucleus conductivity

© 2011 Colin Chung, Martin Waterfall, Steve Pells, Anoop Menachery, Stewart Smith, Ronald Pethig, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
Biodielectrics, Cytoplasm Conductivity, Dielectrophoresis, Myeloma Cells, Nucleus conductivity