<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Metastasis is responsible for most cancer-related death, and the metastatic spread of neoplastic cells may be related to the ability of migration and invasion. Chemokine receptor 9 (CCR9) plays an important role in cutaneous melanoma and prostate cancer cells migration and invasion.Objective: Investigate the specific role of the chemokine-ligand (CCR9-CCL25) axis in the development of nonsmall cell lung cancer (NSCLC) metastasis.Methods: Semi-quantitative reverse transcriptase-PCR, western-blot, flow cytometry, migration, and invasion assays were used to examine the function of CCR9 in the NSCLC cells.Results: CCR9 was highly expressed in NSCLC patient cancer tissue. In addition, in vitro migration and invasion studies on human bronchial epithelial cells of the BEAS-2B and human squamous lung cancer cell lines NCI-H157 showed that migration in response to the CCL25 was inhibited by CCR9 antibody.Conclusion: CCR9 might play an important role in the migration and invasion of the NSCLC cells.</abstract>

Background: Metastasis is responsible for most cancer-related death, and the metastatic spread of neoplastic cells may be related to the ability of migration and invasion. Chemokine receptor 9 (CCR9) plays an important role in cutaneous melanoma and prostate cancer cells migration and invasion.Objective: Investigate the specific role of the chemokine-ligand (CCR9-CCL25) axis in the development of nonsmall cell lung cancer (NSCLC) metastasis.Methods: Semi-quantitative reverse transcriptase-PCR, western-blot, flow cytometry, migration, and invasion assays were used to examine the function of CCR9 in the NSCLC cells.Results: CCR9 was highly expressed in NSCLC patient cancer tissue. In addition, in vitro migration and invasion studies on human bronchial epithelial cells of the BEAS-2B and human squamous lung cancer cell lines NCI-H157 showed that migration in response to the CCL25 was inhibited by CCR9 antibody.Conclusion: CCR9 might play an important role in the migration and invasion of the NSCLC cells.

Chemokine receptor 9 high-expression involved in the migration and invasion of the non-small-cell lung cancer cells

Department of Thoracic Cardiovascular Surgery, People’s Hospital, Wuhan University, Wuhan 430060,

Department of Immunology, College of Basic Medical Sciences, Wuhan University, Wuhan 430071,

Correspondence to: Department of Thoracic Cardiovascular Surgery, People’s Hospital, Wuhan University, 238 Jiefang Rd, Wuhan 430060, PR. China

Department of Thoracic Cardiovascular Surgery, People’s Hospital, Nanning, Guangxi Zhuang Autonomous Region,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Metastasis is responsible for most cancer-related death, and the metastatic spread of neoplastic cells may be related to the ability of migration...

Chemokine receptor 9 high-expression involved in the migration and invasion of the non-small-cell lung cancer cells

Article Category: Original article

Online veröffentlicht: 04. Feb. 2017

Seitenbereich: 69 - 76

DOI: https://doi.org/10.5372/1905-7415.0501.008

Schlüsselwörter
CCR9, CCL25, non-small cell lung cancer, migration, invasion

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Chemokine receptor 9 high-expression involved in the migration and invasion of the non-small-cell lung cancer cells

Article Category: Original article

Online veröffentlicht: 04. Feb. 2017

Seitenbereich: 69 - 76

DOI: https://doi.org/10.5372/1905-7415.0501.008

SchlüsselwörterCCR9, CCL25, non-small cell lung cancer, migration, invasion

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Schlüsselwörter
CCR9, CCL25, non-small cell lung cancer, migration, invasion