<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> In the present study, the presence of <italic>flaA, cadF, cdtB</italic>, and <italic>iam </italic>genes of <italic>Campylobacter </italic>sp. were analysed using PCR. Material for analyses comprised 100 <italic>Campylobacter </italic>sp. isolates obtained from healthy broiler chickens, fatteners, and calves, among which 84 isolates were ascribed to <italic>Campylobacter jejeuni </italic>and 16 to <italic>Campylobacter coli</italic>. All isolates (100%) had the <italic>cadF </italic>gene responsible for adhesion and the <italic>flaA </italic>gene determining the motility of the analysed bacteria. The frequency of occurrence of the <italic>cdtB</italic> gene responsible for the production of the cytolethal distending toxin (CDT) was determined to be high (98.6% in broiler chickens, 75% in fatteners, 62.5% in calves). In case of the <italic>iam </italic>gene, the highest frequency was recorded in <italic>Campylobacter </italic>sp. isolated from broiler chickens (84.7%), while in strains collected from fatteners and calves it was lower, amounting to 41.7% and 18.8%, respectively.</p>
</abstract>

In the present study, the presence of flaA, cadF, cdtB, and iam genes of Campylobacter sp. were analysed using PCR. Material for analyses comprised 100 Campylobacter sp. isolates obtained from healthy broiler chickens, fatteners, and calves, among which 84 isolates were ascribed to Campylobacter jejeuni and 16 to Campylobacter coli. All isolates (100%) had the cadF gene responsible for adhesion and the flaA gene determining the motility of the analysed bacteria. The frequency of occurrence of the cdtB gene responsible for the production of the cytolethal distending toxin (CDT) was determined to be high (98.6% in broiler chickens, 75% in fatteners, 62.5% in calves). In case of the iam gene, the highest frequency was recorded in Campylobacter sp. isolated from broiler chickens (84.7%), while in strains collected from fatteners and calves it was lower, amounting to 41.7% and 18.8%, respectively.

Monitoring of Selected Genes in Campylobacter Jejuni and Campylobacter Coli Isolates from Domestic Animals

Department of General and Environmental Microbiology

Department of Biochemistry and Biotechnology

Journal of Veterinary Research

In the present study, the presence of flaA, cadF, cdtB, and iam genes of Campylobacter sp. were analysed using PCR. Material for analyses comprised 100...

Monitoring of Selected Genes in Campylobacter Jejuni and Campylobacter Coli Isolates from Domestic Animals

Online veröffentlicht: 17. Jan. 2013

Seitenbereich: 283 - 286

DOI: https://doi.org/10.2478/v10213-012-0051-z

Schlüsselwörter
domestic animals, Campylobacter jejuni, Campylobacter coli, genes

This content is open access.

Monitoring of Selected Genes in Campylobacter Jejuni and Campylobacter Coli Isolates from Domestic Animals

Online veröffentlicht: 17. Jan. 2013

Seitenbereich: 283 - 286

DOI: https://doi.org/10.2478/v10213-012-0051-z

Schlüsselwörterdomestic animals, Campylobacter jejuni, Campylobacter coli, genes

This content is open access.

Schlüsselwörter
domestic animals, Campylobacter jejuni, Campylobacter coli, genes