<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>In vitro neurotoxicity testing and toxicity effect quantification plays an important role in many disciplines of biomedicine as an alternative to in vivo methods. The principle of the majority of in vitro methods corresponds to the basic concept of biosensors, i.e. measured quantity is by means of biological sensing element transformed to physical quantity easily measurable by electrical methods of measurement. Two types of biosensors suitable for neurotoxicity measurements are described in the paper. A common feature for both types is application of a living organism as biological sensing element. In the first type of biosensor the morphology of cell is evaluated using image processing methods known as videometry. In the second type of biosensors the electrical impedance of cells using an improved version of the ECIS (Electric Cell-substrate Impedance Sensing) method is a measure of toxicity effects. The results of experiments with biosensors using videometry and a proposal for improvements of ECIS based biosensors are included in the paper.</p></abstract>

In vitro neurotoxicity testing and toxicity effect quantification plays an important role in many disciplines of biomedicine as an alternative to in vivo methods. The principle of the majority of in vitro methods corresponds to the basic concept of biosensors, i.e. measured quantity is by means of biological sensing element transformed to physical quantity easily measurable by electrical methods of measurement. Two types of biosensors suitable for neurotoxicity measurements are described in the paper. A common feature for both types is application of a living organism as biological sensing element. In the first type of biosensor the morphology of cell is evaluated using image processing methods known as videometry. In the second type of biosensors the electrical impedance of cells using an improved version of the ECIS (Electric Cell-substrate Impedance Sensing) method is a measure of toxicity effects. The results of experiments with biosensors using videometry and a proposal for improvements of ECIS based biosensors are included in the paper.

Tissue Morphology and Cell Impedance Based Biosensors for Toxicity Testing

Faculty of Electrical Engineering, CTU Prague, Czech Republic

Measurement Science Review

{"article-title":"Tissue Morphology and Cell Impedance Based Biosensors for Toxicity Testing"}

In vitro neurotoxicity testing and toxicity effect quantification plays an important role in many disciplines of biomedicine as an alternative to in vivo...

Tissue Morphology and Cell Impedance Based Biosensors for Toxicity Testing

Online veröffentlicht: 03. Sept. 2009

Seitenbereich: 105 - 108

DOI: https://doi.org/10.2478/v10048-009-0017-3

Schlüsselwörter
In vitro neurotoxicity testing, Biosensors, Impedance of cells, ECIS method

This content is open access.

Tissue Morphology and Cell Impedance Based Biosensors for Toxicity Testing

Online veröffentlicht: 03. Sept. 2009

Seitenbereich: 105 - 108

DOI: https://doi.org/10.2478/v10048-009-0017-3

SchlüsselwörterIn vitro neurotoxicity testing, Biosensors, Impedance of cells, ECIS method

This content is open access.

Schlüsselwörter
In vitro neurotoxicity testing, Biosensors, Impedance of cells, ECIS method