<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>In an attempt to investigate the genetic and biochemical causes underlying resistance to anthelmintics, the trichostrongylid nematode Haemonchus contortus was examined by an allozymic analysis of nine candidate geneenzyme systems. Two strains resistant to benzimidazoles, two multidrug-resistant strains, and three strains susceptible to anthelmintics isolated from three continents were examined by isoelectric focusing. Two enzymes (mannose phosphate isomerase and peptidase-D) had polymorphic patterns. The peptidase-D zymograms indicated an association with the selection for resistance. Susceptible isolates gave more complex patterns due to the formation of a heterodimer controlled by the two active loci, whereas in resistant isolates, only a single locus with one electromorph was responsible for the profiles. A possible link between dipeptidases and the acquisition of resistance through the turnover of proteinrich collagen, and changes in membrane-associated genes that influence drug efflux, are discussed.</p></abstract>

In an attempt to investigate the genetic and biochemical causes underlying resistance to anthelmintics, the trichostrongylid nematode Haemonchus contortus was examined by an allozymic analysis of nine candidate geneenzyme systems. Two strains resistant to benzimidazoles, two multidrug-resistant strains, and three strains susceptible to anthelmintics isolated from three continents were examined by isoelectric focusing. Two enzymes (mannose phosphate isomerase and peptidase-D) had polymorphic patterns. The peptidase-D zymograms indicated an association with the selection for resistance. Susceptible isolates gave more complex patterns due to the formation of a heterodimer controlled by the two active loci, whereas in resistant isolates, only a single locus with one electromorph was responsible for the profiles. A possible link between dipeptidases and the acquisition of resistance through the turnover of proteinrich collagen, and changes in membrane-associated genes that influence drug efflux, are discussed.

Allozyme analysis of Haemonchus contortus resistant and susceptible to anthelmintics, with an indication of dipeptidases associated with resistance

Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovakia

Department of Biology and Biochemistry, University of Bath, Bath, BA2 7AY, UK

Department of Infectious Diseases and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, 30602, USA

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In an attempt to investigate the genetic and biochemical causes underlying resistance to anthelmintics, the trichostrongylid nematode Haemonchus contortus was...

Allozyme analysis of Haemonchus contortus resistant and susceptible to anthelmintics, with an indication of dipeptidases associated with resistance

Online veröffentlicht: 03. Aug. 2012

Seitenbereich: 128 - 133

DOI: https://doi.org/10.2478/s11687-012-0027-9

Schlüsselwörter
Haemonchus, resistance, peptidase, strain

© 2012 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Allozyme analysis of Haemonchus contortus resistant and susceptible to anthelmintics, with an indication of dipeptidases associated with resistance

Online veröffentlicht: 03. Aug. 2012

Seitenbereich: 128 - 133

DOI: https://doi.org/10.2478/s11687-012-0027-9

SchlüsselwörterHaemonchus, resistance, peptidase, strain

© 2012 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
Haemonchus, resistance, peptidase, strain